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The type of DSB by direct action of X-rays is different comparison with the DSB induced by indirect action
https://repo.qst.go.jp/records/70250
https://repo.qst.go.jp/records/70250da78adfb-07bd-405f-a751-056b51d4bf51
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-09-22 | |||||
| タイトル | ||||||
| タイトル | The type of DSB by direct action of X-rays is different comparison with the DSB induced by indirect action | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Hirayama, Ryoichi
× Hirayama, Ryoichi× Ito, Atsushi× Matsumoto, Yoshitaka× Uzawa, Akiko× Noguchi, Miho× Okayasu, Ryuichi× Furusawa, Yoshiya× 平山 亮一× 伊藤 敦× 松本 孔貴× 鵜澤 玲子× 岡安 隆一× 古澤 佳也 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We examined the contributions of OH radical mediated indirect action of X-ray on cell killing of CHO cells to investigate whether the lethal damage by direct or indirect actions would correlate with DSB reparable capacities using NHEJ-deficient cells (xrs6). The contribution of indirect action on cell killing can be estimated from the maximum degree of protection by DMSO, and that for CHO and xrs6 cells were 77% and 49%, respectively. The percentages of living cells of CHO and xrs6 cells after 2 Gy were 38.4% and 1.0%, respectively. Fractional cell killing by direct action were 14.4% and 50.5%, and that by indirect action were 47.2% and 48.5%, respectively. These results imply that the lethal damage induced by direct action is mostly repaired by NHEJ repair pathway. HR repair system can operate on DSBs induced by indirect action of X-rays. However, xrs6 cells with absolute dependency on the HR were unable to rejoin the DSB induced by direct action leading to more pronounced cell killing than in CHO cells which have two complementary repair pathways. In other words, HR repair system could repair the DSB induced by indirect action in place of NHEJ repair system, although HR repair system could not repair sufficiently the DSB by direct action in place of NHEJ repair system. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 38th Annual Meeting of the European Radiation Research Society | |||||
| 発表年月日 | ||||||
| 日付 | 2010-09-09 | |||||
| 日付タイプ | Issued | |||||
