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Noninvasive assessment of regulable transferred-p53 gene expression and evaluation of therapeutic response with FDG-PET in tumor model
https://repo.qst.go.jp/records/70243
https://repo.qst.go.jp/records/702435cf354e4-e910-408e-869e-d21fe319df7f
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-09-14 | |||||
| タイトル | ||||||
| タイトル | Noninvasive assessment of regulable transferred-p53 gene expression and evaluation of therapeutic response with FDG-PET in tumor model | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
U, Winn Aung
× U, Winn Aung× Hasegawa, Sumitaka× Koshikawa, Michiko× Tsuji, Atsushi× Sogawa, Chizuru× Sudou, Hitomi× Sugyou, Aya× Koizumi, Mitsuru× Furukawa, Takako× Saga, Tsuneo× Ｕ Ｗｉｎｎ Ａｕｎｇ× 長谷川 純崇× 越川 道子× 辻 厚至× 曽川 千鶴× 須藤 仁美× 須尭 綾× 小泉 満× 古川 高子× 佐賀 恒夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The use of tumor-suppressor gene p53 as an anticancer therapeutic has been vigorously investigated. However, progress has met with limited success to date. Some major drawbacks are the difficulty in achieving controllable and efficient gene transfer as well as in analyzing the transferred gene expression in real time and the treatment response in a timely manner. Thus, development of novel gene transfer vector with a regulative gene expression system coupled with the reporter gene, by which transgene can be monitored simultaneously, is critical. Moreover, noninvasive imaging-based assessment of the therapeutic response to exogenous wild-type p53 gene transfer is crucial for refining treatment protocols. In this study, as a simple preclinical model, we constructed a doxycycline-regulated bidirectional vector harboring a reporter gene encoding red fluorescence protein (RFP) and p53. Then, we determined the controllable and simultaneously coordinated expression of both proteins and the p53-mediated anticancer effects in vitro and in vivo. Next, we evaluated glucose utilization in cells with and without exogenous p53 expression by measuring the cellular uptakes of [14C]FDG, a PET radiotracer analogue, indirectly verified the transferred p53 overexpression in xenograft tumor via simultaneous expression of RFP detectable with an in vivo optical imaging system, and subsequently performed FDG-PET imaging in mice. We observed that cells or tumors with induced p53 overexpression exhibited decreased uptake of [14C]FDG in cellular assay and [18F]FDG in PET imaging. Thus, by coupling with novel bidirectional vector, controllable p53 transfer and the assumption that FDG-PET could be used as a surrogate imaging tool to assess the therapeutic response to p53 gene therapy was evidently confirmed, and this may favorably influence the improvement of p53 gene therapy. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | World Molecular Imaging Congress 2010 | |||||
| 発表年月日 | ||||||
| 日付 | 2010-09-11 | |||||
| 日付タイプ | Issued | |||||
