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Hypoxia imaging agent 64Cu-ATSM visualizes cancer stem cell-rich regions within tumors
https://repo.qst.go.jp/records/70197
https://repo.qst.go.jp/records/701979ae116f1-e140-48c2-82f9-1231e14dca01
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-08-03 | |||||
| タイトル | ||||||
| タイトル | Hypoxia imaging agent 64Cu-ATSM visualizes cancer stem cell-rich regions within tumors | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yoshii, Yukie
× Yoshii, Yukie× Waki, Atsuo× Mori, Tetsuya× Yoshii, Hiroshi× Furukawa, Takako× Oh, Myungmi× Kobayashi, Masato× Fujibayashi, Yasuhisa× et.al× 古川 高子 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Objectives: 64Cu-diacetyl-bis (N4-methylthiosemicarbazone) (64Cu-ATSM) is a PET imaging agent targeting tumor hypoxia. It is known that tumor hypoxia assessed by Cu-ATSM is associated with tumor malignant behavior, such as resistance to therapy and metastasis ability. Recent studies demonstrated that the tumor characteristics of therapeutic resistance and metastasis ability would be attributed to so-called cancer stem cells. Based on these understandings, we hypothesized that tumor hypoxic regions visualized by 64Cu-ATSM would contain higher proportions of cancer stem cells than other tumor regions. In this study, we examined distribution of cancer stem cells within tumors and compared it with accumulations of 64Cu-ATSM and 18FDG. \nMethods: Double-tracer autoradiography and immunohistchemistry was performed with mouse colon carcinoma (Colon-26) tumor-bearing mice. In autoradiography, mixture of 74 MBq of 18FDG and 0.37 MBq of 64Cu-ATSM was i.v. injected. CD133 was used as a cancer stem cell marker, which reflected typical characteristics of cancer stem cells in Colon-26 cells. \nResults: Percentage of CD133+ cells was high in order of 64Cu-ATSM high uptake regions >64Cu-ATSM middle uptake regions > 64Cu-ATSM low uptake regions > 18FDG high uptake regions within Colon-26 tumors. The density of CD133+ cells was 11-fold higher in 64Cu-ATSM high uptake regions than in 18FDG high uptake regions. \nConclusions: 64Cu-ATSM accumulating hypoxic regions matched CD133+ cell-rich regions within Colon-26 tumors. Our findings suggested that 64Cu-ATSM is a potential imaging agent for cancer stem cell-rich regions within tumors. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 2009 SNM Annual Meeting | |||||
| 発表年月日 | ||||||
| 日付 | 2009-06-17 | |||||
| 日付タイプ | Issued | |||||
