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Number of stem-like cells and the genetic susceptibility to mammary carcinogenesis in rats
https://repo.qst.go.jp/records/70185
https://repo.qst.go.jp/records/70185d4a2ef8a-d565-4e4a-9699-0f95734d2fc7
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-07-20 | |||||
| タイトル | ||||||
| タイトル | Number of stem-like cells and the genetic susceptibility to mammary carcinogenesis in rats | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Shimada, Yoshiya
× Shimada, Yoshiya× Nishimura, Mayumi× Imaoka, Tatsuhiko× Kakinuma, Shizuko× Gould, Michael× Clifton, Kelly× 島田 義也× 西村 まゆみ× 今岡 達彦× 柿沼 志津子 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Background: The rat mammary tumor model has been used for the study on the biology of human breast cancer. Genetic predisposition for mammary carcinogenesis plays a significant role in the rats. Female Sprague-Dawley and Wistar-Furth (WF) rats are sensitive to DMBA- and MNU-induced mammary carcinogenesis, but Copenhagen (COP) rats are completely resistant. F1 hybrids (WF x COP) show resistance, suggesting a dominant tumor suppressive trait of the COP background. The underlying genetic components are complex and not completely understood. Stem cells and their immediate progeny are considered as the targets for malignant transformation. To elucidate the cellular basis for resistance to mammary carcinogenesis in COP rats, we performed transplantation assays to examine the number of stem-like cells, previously referred to as clonogens, and their response to cancer promoting condition (glucocorticoid deficiency and high prolactin) in comparison with susceptible WF rats. Materials and methods: Young-adult female WF and COP rats and their F1 hybrids (WF x COP) were used. The number of stem-like cells was determined by a transplantation assay. Two types of donor rats were used: untreated rats and adrenalectomized, pituitary-transplanted rats. Serially diluted monodispersed mammary epithelial cells from donor rats were transplanted into the interscapular fat pad of recipient F1 rats grafted with mammotrophic pituitary tumor cells. Three weeks after transplantation, the fat pads were removed and examined for the presence of alveolar units (AUs) and branching ductal units (DUs) developed at graft sites. The total number of AU- or DU-forming cells per mammary gland was calculated. Results: The total number of AU-forming cells per normal female mammary gland was much smaller in COP than WF, being coincided with tumor susceptibility. However, the number of AU-forming cells of F1 rats was comparable to those of WF, which failed to account for the differential tumor susceptibility between WF and F1. On the other hand, the number of DU-forming cells of F1 was one third of those of WF, in good agreement with their tumor susceptibility. More importantly, DU-forming cells in COP and F1 were not stimulated to expand by glucocorticoid deficiency and high level of prolactin, in contrast with the marked response by the WF cells. Conclusion: The DU-forming cells may be the targets for chemically induced mammary carcinomas. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 21st Meeting of the European Association for Cancer Research | |||||
| 発表年月日 | ||||||
| 日付 | 2010-06-29 | |||||
| 日付タイプ | Issued | |||||
