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A mouse strain that is sensitive for iPS cellgeneration: C57BL/6
https://repo.qst.go.jp/records/70168
https://repo.qst.go.jp/records/701687086a82b-815f-40bd-b393-d033f8be2f13
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-06-22 | |||||
| タイトル | ||||||
| タイトル | A mouse strain that is sensitive for iPS cellgeneration: C57BL/6 | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Jincho, Yuko
× Jincho, Yuko× et.al× 神長 祐子 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | There are many similar aspects between cancer cells and iPS cells (iPSCs), and it is well known that some biological phenomena, including tumorigenesis, depend on the mouse strain. Therefore, we examined six mouse strains including three inbred strains, C3H, 129, and C57BL/6, and found a clear difference in iPS generation between these strains. C57BL/6 was revealed to be a strain of which embryonic fibroblasts (MEF) cause iPSC with a high incidence rate, and an efficient iPS cells generation without c-Myc transduction was also shown in the strain. \nHere we succeeded in generating iPSCs from C57BL/6 mice MEF using the virus-free and c-Myc-free gene transduction system with 2A-3F vector that contains Oct3/4, Klf4 and Sox2 genes. As expected, several integration-free colonies were included among these iPS clones, and such clones were indistinguishable from other iPS cells which have been established with various methods thus far, using retrovirus-mediated gene transduction system and with the defined four factors including c-Myc. In addition to the gene expression profiling analysis, their developmental potential was examined by the teratomas formation and chimera mice formation tests. \nFurthermore, we also succeeded in generating iPSCs with three factors excluding Sox2 from the MEF of C57BL/6 with retro virus-mediated gene transduction system. Characterization of the iPSCs (-Sox2) was performed with the same analyses for the iPSCs generated by 2A-3F plasmid, and then their quality was verified. \nVarious cells, including embryonic fibroblasts, prepared from C57BL/6 strain would allow us to conduct more in-depth and interesting investigations on the iPSC generation mechanism. In addition, C57BL/6 is suitable for longitudinal studies such as cancer study, and also suitable and powerful for genetic studies because C57BL/6 is the mouse strain that was selected for the whole genome sequencing project. Finally, our present results may suggest that c-Myc- and genome integration-free iPS cells can be established in a portion of human population, since mouse strain difference conceptually corresponds to the difference among human individuals. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | ISSCR 8th Annual Meeting | |||||
| 発表年月日 | ||||||
| 日付 | 2010-06-19 | |||||
| 日付タイプ | Issued | |||||
