WEKO3
アイテム
Induction of stress-responsive genes by carbon-ion irradiation in sequential biopsy specimens from patients with cervical cancer.
https://repo.qst.go.jp/records/70102
https://repo.qst.go.jp/records/70102ae1cdfea-a22a-4ad4-810b-1501d89c3b11
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-05-25 | |||||
| タイトル | ||||||
| タイトル | Induction of stress-responsive genes by carbon-ion irradiation in sequential biopsy specimens from patients with cervical cancer. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Fujita, Hidetoshi
× Fujita, Hidetoshi× Iwakawa, Mayumi× Nakawatari, Miyako× Imadome, Kaori× Nakamura, Etsuko× Fujita, Tomoko× Kato, Shingo× Ohkubo, Yu× Imai, Takashi× 藤田 英俊× 岩川 眞由美× 中渡 美也子× 今留 香織× 中村 悦子× 藤田 知子× 加藤 眞吾× 大久保 悠× 今井 高志 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Propose: Irradiation of carbon-ion (C-ion) leads to the formation of severe DNA damage without dependency on the cell cycle phase and oxygen status. However, the molecular mechanisms underlying the therapeutic response to C-ion are not fully understood. In this study, we investigated the molecular pathways induced by C-ion radiotherapy using sequentially taken biopsy samples of cervical cancers to understand the biological mechanism specific to C-ion radiotherapy. Methods: Biopsy specimens were obtained from 60 patients with cervical cancer, before and during fractionated C-ion radiotherapy. C-ion treatment was initiated with a fraction dose of 2.2 GyE and total dose was 71.2- 74.4 GyE. A second biopsy was taken 1 day after the start of radiation. Transcriptome analysis was performed using single-color oligo-microarrays consisting of 44 K human sequences for biopsy samples from 22 patients. Expression values of genes before and during radiotherapy were compared and the statistical analysis was used with the Wilcoxon signed rank test (p < 0.001). The changes of gene expression were confirmed by Real-time PCR analysis, and further immunohistological analysis was performed to confirm the expression and localization of target proteins using newly enrolled 38 patients. Results: Two hundred and twenty genes were identified as C-ion responded genes (CRGs) in cervical cancers under our microarray-analysis conditions. We found three new CRGs in addition to the previously reported ionizing radiation-induced genes such as CDKN1A, BAX, Fas, and DDB2. The p53-target genes such as MDM2, TRIAP1 and GADD45 were also responded to C-ion in the cervical cancers. Comparison of the expression profiles of the human cervical cancers with those of the experimental murine tumors (Imadome et al. Cancer Biol. Ther. 7: 208 – 217, 2008) showed that the genes categorized into the cell-communication and/or the stress-response were commonly involved in both the expression profiles after C-ion irradiation. Conclusions: Genes responded to C-ion irradiation in human cervical cancers were identified. These genes were mainly involved in cell communication or stress response pathways. Our findings may lead to a better understanding the molecular mechanisms underlying the therapeutic response to C-ion. |
|||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | PTCOG 49 (49th Annual Meeting of the Particle Therapy Co-Operative Group) | |||||
| 発表年月日 | ||||||
| 日付 | 2010-05-22 | |||||
| 日付タイプ | Issued | |||||
