WEKO3
アイテム
First in vitro radiobiological characterizations of cells from chordoma origin
https://repo.qst.go.jp/records/70085
https://repo.qst.go.jp/records/700858256f0d3-7483-4a1c-88cb-edd6df915f24
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-04-23 | |||||
| タイトル | ||||||
| タイトル | First in vitro radiobiological characterizations of cells from chordoma origin | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Kato, Takamitsu
× Kato, Takamitsu× Tsuda, Akihisa× Fujimori, Akira× Kamada, Tadashi× Tsujii, Hirohiko× Okayasu, Ryuichi× 加藤 宝光× 津田 晃久× 藤森 亮× 鎌田 正× 辻井 博彦× 岡安 隆一 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Although chordoma, a rare bone cancer, has been treated with surgery and/or radiation including heavy ions, few in vitro characterizations of chordoma cells are available. The main reason for this is the extremely long doubling time associated with the only recognized cell line, U-CH1. Here, we derived a cell line (designated as U-CH1-N) from U-CH1 and made the in vitro characterization possible, and results with U-CH1-N were compared with those with HeLa (cervical cancer) and U87-MG (glioblastoma) cells. The cell doubling times for HeLa, U87-MG and U-CH1-N were 18 h, 24 h and 3 days respectively. U-CH1-N had the most DNA content among the three, was near tetraploid, and showed an abnormal karyotype. U-CH1-N and U87-MG were more sensitive to x-irradiation than HeLa cells. Heavy ion irradiation more efficiently killed all three cell lines than x-rays did. Relative biological effectiveness (RBE) at 10% survival for U87-MG and HeLa cells was about 2.5 for 70keV/um carbon ions and 3 for 200keV/um iron ions, while for U-CH1-N it was 2.5 for carbon and 4 for iron ions. Four different chemical agents producing various DNA lesions were used for further characterization of these cells. Although camptothecin, mitomycin C or cisplatin did not reveal strong cytotoxity to U-CH1-N compared with other cells, bleomycin, which produces DNA strand breaks, showed marked cytotoxic effect on U-CH1-N. Our data provide the first chronological cell survival information using cells of chordoma origin and also help explain the successful chordoma treatment by heavy ions. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | AACR Annual Meeting 2010 | |||||
| 発表年月日 | ||||||
| 日付 | 2010-04-21 | |||||
| 日付タイプ | Issued | |||||
