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Use of high performance liquid chromatography coupled with isotope ratio mass spectrometry for the measurement of carbon chemical form changes in waterlogged soil

https://repo.qst.go.jp/records/69800
https://repo.qst.go.jp/records/69800
8b1a7429-ee9a-42ba-b5a2-b0ef89ca7baa
Item type 会議発表用資料 / Presentation(1)
公開日 2009-07-21
タイトル
タイトル Use of high performance liquid chromatography coupled with isotope ratio mass spectrometry for the measurement of carbon chemical form changes in waterlogged soil
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_c94f
資源タイプ conference object
アクセス権
アクセス権 metadata only access
アクセス権URI http://purl.org/coar/access_right/c_14cb
著者 Tagami, Keiko

× Tagami, Keiko

WEKO 685326

Tagami, Keiko

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Uchida, Shigeo

× Uchida, Shigeo

WEKO 685327

Uchida, Shigeo

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田上 恵子

× 田上 恵子

WEKO 685328

en 田上 恵子

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内田 滋夫

× 内田 滋夫

WEKO 685329

en 内田 滋夫

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抄録
内容記述タイプ Abstract
内容記述 It is possible to use stable carbon isotopes as a tracer instead of radioactive C-14 to understand the organic compounds fate in the soil environment. In this study, therefore, we used high performance liquid chromatography coupled with isotope ratio mass spectrometry (HPLC-IRMS) to measure the changes of chemical form added to soil.
A soil sample (0.5 g-dry) was added to 5mL of deionized water, and then, a small portion of acetic acid, which was labeled with C-13, was spiked to obtain 2mM carbon concentration in the surface water. At 0, 8, 16, 24, 48, 72 and 168 h after addition of acetic acid, the surface water was collected destructively. Immediately after the solution was passed through a 0.2-um filter, the filtrate was analyzed by HPLC-IRMS. We also kept the filtrates for 6 months at 5C to check the sample stability.
The results showed that the acetic acid was detected for the first 24 h, but could not be detected afterwards. No other chemical form was observed by this HPLC-IRMS system. We assumed that the added carbon would be immobilized by the soil or discharged to the air. Concerning the sample storage period, no data difference between before and after the storage was found.
会議概要(会議名, 開催地, 会期, 主催者等)
内容記述タイプ Other
内容記述 第5回放射線の安全と測定技術に関する国際シンポジウム(ISORD-5)
発表年月日
日付 2009-07-17
日付タイプ Issued
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