WEKO3
アイテム
Detailed analysis of Ikaros mutation in mouse thymic lymphoma induced by simultaneous exposure of X-rays and N-ethyl-N-nitrosourea
https://repo.qst.go.jp/records/69755
https://repo.qst.go.jp/records/69755de4a758c-5cd0-4076-b206-3ca63d19dbcf
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2009-05-25 | |||||
| タイトル | ||||||
| タイトル | Detailed analysis of Ikaros mutation in mouse thymic lymphoma induced by simultaneous exposure of X-rays and N-ethyl-N-nitrosourea | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Amasaki, Yoshiko
× Amasaki, Yoshiko× Hirano, Shinobu× Yamauchi, Kazumi× Nishimura, Mayumi× Imaoka, Tatsuhiko× Shimada, Yoshiya× Kakinuma, Shizuko× 甘崎 佳子× 坂入 しのぶ× 山内 一己× 西村 まゆみ× 今岡 達彦× 島田 義也× 柿沼 志津子 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Purpose Radiation carcinogenesis in human is considered as a result of the combined effect of radiation and environment factors. This study aimed to elucidate the mode and mechanism of the combined effect on thymic lymphoma development after simultaneous exposure of X-rays and N-ethyl-N-nitrosourea (ENU). \nMethods Four and eight-week-old female B6C3F1 mice were simultaneously exposed to X-rays (0.2 to 1.0 Gy per week) and ENU (50 to 200 ppm) in drinking water for 4 consecutive weeks for the induction of thymic lymphomas. Ikaros alteration in lymphomas was analyzed by RT-PCR, DNA sequencing and Western blotting. \nResults Combination of low dose of X-rays at dose less than 0.4 Gy per fraction and ENU at 50 ppm did not induce lymphomas. In contrast, combination at higher dose of X-rays and ENU enhanced lymphoma development synergistically. Ikaros is tumor suppressor gene, which is frequently altered in lymphomas by point and frameshift mutations, alternative splicing, transcriptional silencing. The frequency of total Ikaros alteration in the lymphomas after 4-week- and 8-week-old treatment was 50% and 30%, respectively. Among these, point mutation made up 43% and 18%, respectively. The base substitutions of T to C and G to A/T mostly contributed. Interestingly, G to A/T was identified in only lymphomas induced by combined exposure started at 4-week-old. \nConclusion Simultaneous exposure to X-rays and ENU enhanced T to C base substitution, and generated new types of G to A/T. The Ikaros point mutation appeared to be affected by the age-at-exposure. |
|||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 2nd Asian Congress of Radiation Research (ACRR2009) | |||||
| 発表年月日 | ||||||
| 日付 | 2009-05-20 | |||||
| 日付タイプ | Issued | |||||
