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Distinct structural abnormalities of chromosomes 11 and 12 associated with loss of heterozygosity in X-ray-induced mouse thymic lymphomas
https://repo.qst.go.jp/records/69516
https://repo.qst.go.jp/records/69516cca14ea8-7046-4641-83f0-500a6e990d13
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-10-08 | |||||
| タイトル | ||||||
| タイトル | Distinct structural abnormalities of chromosomes 11 and 12 associated with loss of heterozygosity in X-ray-induced mouse thymic lymphomas | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Nakata, Akifumi
× Nakata, Akifumi× Yoshida, Mitsuaki× Akiyma, Miho× Kakinuma, Shizuko× Sado, Toshihiko× Nishimura, Mayumi× Shimada, Yoshiya× 中田 章史× 吉田 光明× 穐山 美穂× 柿沼 志津子× 西村 まゆみ× 島田 義也 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Mouse thymic lymphomas (TLs) are one of the classical models for spontaneously developed, and radiation- or chemical carcinogen-induced malignancy and are a model for mechanistic study of human acute lymphoblastic leukemia. In the early studies of chromosome abnormality, trisomy of chromosome 15 was identified frequently in TLs and seemed to be the specific change associated with the development of this malignancy. Recent studies have demonstrated that radiation-induced TLs show frequent loss of heterozygosity (LOH) on chromosomes 4 (p15/p16), 11 (Ikaros), 12 (Bcl11b), 19 (Pten) and X. Since cytogenetic analysis of murine thymic lymphoma has not been studied for these two decades, we think it worthy to re-evaluate cytogenetics of TL using both classical cytogenetic method and molecular cytogenetics using FISH with chromosome-specific probes focusing on the region with frequent LOH or loss and gain of genetic materials. In this study, C57BL/6 (B6), C3H and F1 (B6 x C3H) mice were used. TLs were induced by whole-body irradiation with 4- to 5-week-old mice exposing to X-irradiation (1.2 or 1.6 Gy/exposure) once a week for four consecutive weeks using a PANTAK X-ray generator at 0.7 Gy/min (200 kVp, 20 mA, with filters of 0.5 mm Cu and 0.5 mm Al). The mice were observed for 3 months or until moribund and were sacrificed under ether anesthesia to remove the enlarged thymus. Chromosomally abnormal cells were present in 25 TLs examined (25/26, 96%). The most frequent abnormality was trisomy or partial trisomy of chromosome 15 (16/26, 62%), being consistent with previous studies. Structural abnormalities of chromosome 11 with interstitial deletion of the proximal region and chromosome 12 translocations with deletion of the distal region were newly identified in 7 (27%) and 12 (46%) cases, respectively. Monosomy of chromosome X was also found in 4 cases (15%). No apparent aberrations were detected in chromosomes 4 and 19. Only three cases had a reciprocal translocation, t(X;17), t(3;X) and t(1;12). These results indicate that a distinct mechanism contributes to LOH of each tumor suppressor gene in different chromosomal locations. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 13th international Congress of Radiation Research | |||||
| 発表年月日 | ||||||
| 日付 | 2007-07-12 | |||||
| 日付タイプ | Issued | |||||
