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Sensitization of cancer cells to radiation using hybrid nanoparticles: Activation of apoptotic factors
https://repo.qst.go.jp/records/69416
https://repo.qst.go.jp/records/694160d65d758-91ad-4a69-a16d-4fb087b89f29
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-07-29 | |||||
| タイトル | ||||||
| タイトル | Sensitization of cancer cells to radiation using hybrid nanoparticles: Activation of apoptotic factors | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Zhelev, Zhivko
× Zhelev, Zhivko× Bakalova-Zheleva, Rumiana× Aoki, Ichio× Kanno, Iwao× et.al× Ｚｈｅｌｅｖ Ｚｈｉｖｋｏ× バカロバ ルミアナ× 青木 伊知男× 菅野 巖 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Sensitization of cancer cells to radiation using hybrid nanoparticles: Activation of apoptotic factors Z. Zhelev,1 R. Bakalova,1 I. Aoki,1 M. Mileva,2 V. Gadjeva,3 I. Kanno1 1Molecular Imaging Center, National Institute of Radiological Sciences (NIRS), Chiba, Japan 2Department of Physics and Biophysics, Medical University, Sofia, Bulgaria 3Medical Faculty, Thracian University, Stara Zagora, Bulgaria \nIntroduction: Nanotechnology-based tools and techniques are rapidly emerging in the field of molecular imaging diagnostics and targeted drug/gene delivery, a field that is expected to generate many innovations and play a crucial future role in medicine. In the past 3 years, several leading groups in "nanomedicine" have reported that hybrid nanoparticles, consisting of semiconductors quantum dots (QDs) and conventional photosensitizers (e.g., phthalocyanines, eosins, natural pigments, etc.) or QDs and X-ray photosensitizers, in radiation-induced photodynamic therapy of cancer. The nanoparticles can also have a promising application be conjugated with cancer-selective ligands that should minimize side-effects on normal cells and tissues. In this study, we describe the selective sensitization of cancer cells to laser irradiation, using several QD-based hybrid nanoparticles, conjugated with cluster-of-differentiation antibodies specific for leukemia cells. Methods: The experiments were performed on cultured cells, derived from patients with acute lymphoblastic leukemia or chronic myeloid leukemia, as well as on normal lymphocytes, derived from healthy volunteers. The cells were incubated with QD probes over different time-intervals (up to 48 hours). Interaction of the QDs with the cells was detected by fluorescent confocal microscopy. After incubation, the cells were subjected to laser irradiation (red light). The cell viability was analyzed before and after irradiation, using flow cytometry. The apoptosis was analyzed using fluorescein-labeled Annexin-V, activation of caspase cascade, and DNA fragmentation tests. The generation of reactive oxygen species was detected using EPR spectroscopy. Results: Fluorescent confocal imaging demonstrated that QD-based hybrid nanoparticles interact specifically with leukemia cells, but do not interact with normal lymphocytes. Without laser irradiation, the nanoparticles did not affect significantly cell viability. However, the viability of QD-treated leukemia cells markedly decreased (~25-40%, depending on the chemical structure of the QD probe) after laser irradiation, while the viability of normal lymphocytes remained at the control level. Acceleration of free radical generation and induction of apoptosis (with activation of caspase enzymes and DNA-fragmentation) were also detected in the cancer cells. In normal lymphocytes, we observed only a slight reversible expression of phosphatidylserine on the cell surface (detected by Annexin-V) after QD treatment and subsequent laser irradiation. Conclusions: The results suggest that non-coated QD-based hybrid nanoparticles manifest target-selective laser-induced cytotoxicity due to specific interaction with leukemia cells. The mechanism of QD induced cancer cell death is due to acceleration of free radical generation and induction of apoptosis. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | the 5th International Symposium on Nanoscience & Nanotechnologies | |||||
| 発表年月日 | ||||||
| 日付 | 2008-07-16 | |||||
| 日付タイプ | Issued | |||||
