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Contribution of indirect action of X-irradiation under oxic and hypoxic conditions in different radiosensitivity cells

https://repo.qst.go.jp/records/69356
https://repo.qst.go.jp/records/69356
bca32a0c-cb2b-41e8-9b85-e15e6efe5528
Item type 会議発表用資料 / Presentation(1)
公開日 2008-06-16
タイトル
タイトル Contribution of indirect action of X-irradiation under oxic and hypoxic conditions in different radiosensitivity cells
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_c94f
資源タイプ conference object
アクセス権
アクセス権 metadata only access
アクセス権URI http://purl.org/coar/access_right/c_14cb
著者 Hirayama, Ryoichi

× Hirayama, Ryoichi

WEKO 680657

Hirayama, Ryoichi

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Furusawa, Yoshiya

× Furusawa, Yoshiya

WEKO 680658

Furusawa, Yoshiya

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Ito, Atsushi

× Ito, Atsushi

WEKO 680659

Ito, Atsushi

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Noguchi, Miho

× Noguchi, Miho

WEKO 680660

Noguchi, Miho

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Matsumoto, Yoshitaka

× Matsumoto, Yoshitaka

WEKO 680661

Matsumoto, Yoshitaka

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Uzawa, Akiko

× Uzawa, Akiko

WEKO 680662

Uzawa, Akiko

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Ando, Koichi

× Ando, Koichi

WEKO 680663

Ando, Koichi

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Okayasu, Ryuichi

× Okayasu, Ryuichi

WEKO 680664

Okayasu, Ryuichi

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平山 亮一

× 平山 亮一

WEKO 680665

en 平山 亮一

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古澤 佳也

× 古澤 佳也

WEKO 680666

en 古澤 佳也

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伊藤 敦

× 伊藤 敦

WEKO 680667

en 伊藤 敦

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松本 孔貴

× 松本 孔貴

WEKO 680668

en 松本 孔貴

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鵜澤 玲子

× 鵜澤 玲子

WEKO 680669

en 鵜澤 玲子

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安藤 興一

× 安藤 興一

WEKO 680670

en 安藤 興一

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岡安 隆一

× 岡安 隆一

WEKO 680671

en 岡安 隆一

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抄録
内容記述タイプ Abstract
内容記述 We examined the contribution of indirect action of X-irradiation under oxic and hypoxic conditions in cell killing of a CHO line and its NHEJ-defective xrs6 mutant line (a Ku80 mutant of CHO). X-irradiation was performed at a dose rate of 4.9 Gy/min using a generator operating at 200 kVp and 20 mA. For oxic and hypoxic conditions, irradiation chambers were flushed for more than 1 hour with air + 5% CO2 and 95% N2 + 5% CO2, respectively, prior to irradiation at room temperature. Protective effect of DMSO which specifically scavenges OH radicals was evaluated from cell survivals. Fractions of cell killing by the OH mediated indirect action were calculated by the method of Shinohara et al. (Acta Oncol., 35, 869-75. 1996). The fractions for CHO were 80% and 60% under oxic and hypoxic conditions, respectively. The fractions for xrs6 were 50% and 30% under oxic and hypoxic conditions, respectively. Thus, our data indicate that the indirect action of OH radicals in cell killing is dependent of the oxygen concentration and the repair capacity of the cells. However, radiolysis study of liquid water demonstrated previously that the G(OH) value was independent of oxygen concentration (C. V. Sonntag, The Chemical Basis of Radiation Biology, 33. 1987).
When the repair proficient and deficient cells were compared, the contribution of the indirect action in cell killing was higher for the former than for the latter. In other words, the contribution of direct action was more pronounced in xrs6 cells. When the actual numbers of damages contributing for the cell killing were estimated, it was found that the repair deficient cells were more sensitive to the damage produced under hypoxia. It is our hypothesis that DNA damage produced under hypoxia is more complex and less likely to be repaired. This type of damage is especially problematic for NHEJ deficient xrs6 cells. Taken together, DNA damage due to direct action of X-rays was more akin to those by high LET radiations.
会議概要(会議名, 開催地, 会期, 主催者等)
内容記述タイプ Other
内容記述 10th International Workshop Radiation Damage to DNA
発表年月日
日付 2008-06-13
日付タイプ Issued
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