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Anti-oxidative enzyme catalase controls proliferation of HaCaT cells through the regulation of heparan sulfate 2-O-sulfotransferase
https://repo.qst.go.jp/records/69252
https://repo.qst.go.jp/records/69252f999fc92-684c-4276-b2c9-1858ceb134a3
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-12-27 | |||||
| タイトル | ||||||
| タイトル | Anti-oxidative enzyme catalase controls proliferation of HaCaT cells through the regulation of heparan sulfate 2-O-sulfotransferase | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Nakayama, Fumiaki
× Nakayama, Fumiaki× Hagiwara, Akiko× Akashi, Makoto× 中山 文明× 萩原 亜紀子× 明石 真言 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Heparan sulfate (HS) chains can interact with a variety of growth factors possessing heparin-binding domains, such as fibroblast growth factors (FGFs), enabling them to transfer to their high-affinity signaling receptors on the cell surface. In this study, we demonstrated the correlation between the expression of anti-oxidative enzymes and the HS chain in the human HaCaT keratinocyte cell line. The overexpression of catalase induced an increase of anti-HS antibody (10E4) epitope expression in these cells; however the MnSOD transfectant expressed it at the same level as the control cells. Western blotting showed that the smeared bands of HSPG were obviously shifted to higher-molecular-weight in the catalase transfectants owing to glycosylation. The levels of glycosyltransferases (XT-II, EXTL2, GLCE, HS2ST1 and HS6ST1) transcripts were significantly increased in the transfectants. In contrast, siRNA-mediated repression of catalase decreased 10E4 epitope expression, the transcript level of HS2ST1, and proliferation of HaCaT cells; however it did not significantly down-regulate the transcript level of HS6ST1. In addition, siRNA-mediated repression of HS2ST1 decreased HaCaT cell proliferation, although repression of HS6ST1 did not reduce it. These findings suggest that catalase may be able to modify the HS chains in the keratinocytes through the regulation of HS2ST1 to control HaCaT cell proliferation. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 37th Annual ESDR Meeting 2007 | |||||
| 発表年月日 | ||||||
| 日付 | 2007-09-08 | |||||
| 日付タイプ | Issued | |||||
