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Vascular dimension, blood plasma speed and arteriovenous transit time in anesthetized rat cortex
https://repo.qst.go.jp/records/69209
https://repo.qst.go.jp/records/6920953e04af9-83e0-4e29-a76a-89548dac8e3e
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-12-03 | |||||
| タイトル | ||||||
| タイトル | Vascular dimension, blood plasma speed and arteriovenous transit time in anesthetized rat cortex | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Masamoto, Kazuto
× Masamoto, Kazuto× Obata, Takayuki× Kanno, Iwao× 正本 和人× 小畠 隆行× 菅野 巖 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The vascular dimensions and blood flow structure were systematically characterized along the branching orders of the cortical vessel trees in anesthetized rat cortex in vivo. The animals (300-340 g Sprague-Dawley rats, N = 4) were anesthetized with either isoflurane (~1.4%) or alpha-chloralose (45 mg/kg/h, i.v.). The portion of the skull over the somatosensory area was removed while the dura was kept intact. FITC-dextran (MW = 7000) was intravenously injected, and the cortical vasculature was visualized in vivo with confocal laser-scanning microscopy (488-nm excitation) or multi-photon excitation fluorescent microscopy (900-nm excitation). The lumen diameter of the vessels was measured from the arterial to venous compartments, including the capillaries distributed beneath the cortical surface at depths of up to~0.4 mm. The size of the major cerebral artery (A1) was 97 +/- 17 mum under isoflurane anesthesia, but was observed to decrease remarkably after injection of alpha-chloralose to 75 +/- 15 mum. Similar shrinkage of the lumen diameter was observed for the second (A2) and third (A3) branched arteries; 66 and 45 mum under isoflurane vs. 44 and 35 mum under alpha-chloralose. However, no clear difference in lumen size for either anesthetic was found for the fourth (A4) and fifth (A5) branched arterioles (31 and 23 mum under isoflurane vs. 30 and 21 mum under alpha-chloralose), and all venous compartment vessels (V5, V4, V3, and V2); 22, 34, 85, and 182 mum under isoflurane, and 26, 35, 81, and 175 mum under alpha-chloralose, respectively. The results indicate that the relatively large size of the arteries and arterioles plays a major role in controlling baseline CBF during different anesthetic conditions. Further, with intravenously injected fluorescent microspheres (1 mum in diameter), the blood plasma speed and arteriovenous transit time were evaluated in each cerebrovascular compartment and across the various branching points. We observed similar arteriovenous transit times under either anesthetic condition and similar blood speed profiles. These findings further support the conclusion that the arterial regulation of blood volume (CBV) plays a key role in determining anesthetic-dependent baseline CBF. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Neuroscience 2007, the 37th annual meeting | |||||
| 発表年月日 | ||||||
| 日付 | 2007-11-07 | |||||
| 日付タイプ | Issued | |||||
