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Stability and reactivity of reaction mixtures containing anitroxyl free radical probe and da hydrogen donor for detection free radical reactions induced by ionizing irradiation
https://repo.qst.go.jp/records/69186
https://repo.qst.go.jp/records/69186e72ce506-9099-4365-aa7f-a35f9331806d
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-11-22 | |||||
| タイトル | ||||||
| タイトル | Stability and reactivity of reaction mixtures containing anitroxyl free radical probe and da hydrogen donor for detection free radical reactions induced by ionizing irradiation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Matsumoto, Kenichiro
× Matsumoto, Kenichiro× et.al× 松本 謙一郎 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Oxygen effect has been a problem in a radiation therapy using carbon ion beam. Free radical reaction/generation may not simply depend linearly on the dose and LET when heavy ion beam was irradiated to a biological samples. A method to quantify amount of free radical reaction/generation in a biological sample is requred. In this study, stability and reactivity of reaction mixtures containing 0.1 mM of a nitroxyl free radical probe, i.e. 4-hydroxyl-TEMPO (TEMPOL) or 3-carbamoyl-PROXYL (CmP), and 1 M of a hydrogen donor (H-donor), i.e. GSH, NADH, or NADPH were tested. X-band EPR measurements of several reaction mixtures were performed to find a suitable condition to detect free radical reactions using low linear-energy-transfer (LET) irradiation at relatively low dose (< 16 Gy). The EPR signal intensity of nitroxyl radicals were decreased by X-ray irradiation when the hydrogen donor was coexisting in the reaction mixture. It was found that presence of H-donor, such as GSH or NAD(P)H, was indispensable to detect reduction of nitroxyl radicals by a relatively low dose irradiation. It was strongly suggested the formation of ROS, such as ∙OH and/or ∙O2-, in aqueous solution by radiation. The main reaction of NAD(P)H and nitroxyl radicals may be the one-electron reduction of nitroxyl radical to corresponding hydroxylamine, which may be two-step reaction via oxoamonium cation. The reaction of GSH and nitroxyl radicals may have another pass, which may be irreversible. The combination of TEMPOL and GSH was most preferable to detect free radical reaction caused by radiation quantitatively among the conditions tested in this study, because of the sensitivity and irreversibity of the reaction. This combination can also be applied to test effects of other radiation sources, i.e. beta-, and gamma-ray. This method will be applied to estimate free radical reaction caused by heavy-ion beams in a future study. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | ISESS-SEST 2007（International Symposium on Electron Spin scienceおよび第46回スピンサイエンス学会年会合同ミーティング） | |||||
| 発表年月日 | ||||||
| 日付 | 2007-11-09 | |||||
| 日付タイプ | Issued | |||||
