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Endogenous production of TNFalpha regulates apoptosis by irradiation in a human T cell line Jurkat
https://repo.qst.go.jp/records/69157
https://repo.qst.go.jp/records/69157d2178512-b825-4b4c-8616-dfcfdbc415a5
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-11-08 | |||||
| タイトル | ||||||
| タイトル | Endogenous production of TNFalpha regulates apoptosis by irradiation in a human T cell line Jurkat | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Sudo, Makoto
× Sudo, Makoto× Nakayama, Fumiaki× Watanabe, Keiko× Hachiya, Misao× Akashi, Makoto× 須藤 誠× 中山 文明× 渡辺 恵子× 蜂谷 みさを× 明石 真言 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Irradiation causes various types of cell damage including DNA, stimulates the generation of reactive oxygen species (ROS), and activates multiple signal pathways in cells. Previously, we showed that irradiation markedly increased the tumor necrosis factor alpha (TNFalpha) production in cells. TNFalpha has both apoptotic and anti-apoptotic properties depending on the activation of signaling pathways. To study the role of TNFalpha in irradiation, human T cell line Jurkat cells were transfected with TNFalpha short interfering (si) RNA (siRNA). Studies of competitive PCR showed that an increased level of TNFalpha mRNA was observed immediately after 10 Gy irradiation in control cells; the level reached a maximum at 30 min and then decreased gradually. However, the knock-down of TNFalpha using siRNA significantly abolished the irradiation-induced accumulation of TNFalpha mRNA. We also investigated the mRNA levels of Bax, Bcl-2, and Bcl-XL. Levels of Bcl-2 and Bcl-XL mRNA were similar in control cells and TNFalpha knock-down(k/d) cells. However, the level of Bax mRNA was apparently higher in TNFalpha k/d cells than control cells but irradiation failed to increase the level in both cell lines. On the other hand, staining with Annexin-V and detection of DNA fragmentation showed that irradiation at a dose of 10 Gy induced apoptosis more frequently in the control cells than TNFalpha k/d cells. These results indicate that irradiation induces apoptosis through a pathway requiring TNFalpha production in these cells. Our result also suggest that apoptosis by irradiation occurs independent of the Bax expression. Further studies on the mechanisms are in progress. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 日本分子生物2006フォーラム | |||||
| 発表年月日 | ||||||
| 日付 | 2006-12-08 | |||||
| 日付タイプ | Issued | |||||
