WEKO3
アイテム
Comparative Study on Fluorescence Emission and PET Tracer Uptake in Mouse Mesothelioma Models for Monitoring Growth/Therapeutic Effects
https://repo.qst.go.jp/records/69093
https://repo.qst.go.jp/records/69093a68423d8-880d-4163-acc8-4b26411a1105
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-09-18 | |||||
| タイトル | ||||||
| タイトル | Comparative Study on Fluorescence Emission and PET Tracer Uptake in Mouse Mesothelioma Models for Monitoring Growth/Therapeutic Effects | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Saito, Yuriko
× Saito, Yuriko× Furukawa, Takako× Fujibayashi, Yasuhisa× Saga, Tsuneo× 齋藤 有里子× 古川 高子× 藤林 康久× 佐賀 恒夫 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Various modalities, probes and techniques are now available for in vivo imaging. Each has unique characteristics, and profound understanding of those characteristics is essential for precise comprehension and analysis of the imaging results. Asbestos-induced mesothelioma is a social concern in Japan. Its incidence is anticipated to peak in 10-20 years from now. To facilitate the development of early diagnosis and treatment protocol, we established fluorescent human mesothelioma cell lines, evaluated their tumorigenicity and characterized their in vitro fluorescence emission and PET tracer uptake. Human mesothelioma cell line MSTO-211H was stably transfected to express red fluorescent protein DsRed2 or TurboRFP, and two clones, Ds#4 and Tu#6, emitting bright fluorescence, were selected. Both clones formed subcutaneous and orthotopic tumors in nude mice and the fluorescent intensity measured by IVIS from outside the body correlated with the tumor weight. Under growing conditions in cell culture, strong correlation was observed between the fluorescent intensity and the total and the live cell numbers along with the protein amount. When treated with actinomycin D or cycloheximide, the fluorescent intensity sustained at the initial levels for a couple of days, which correlated better with the total cell number than the live cell number. Uptake of 3H-FLT and 14C-FDG, in contrast, rapidly decreased after the initiation of treatments, dropping more than 30% in 3 hrs, which was much earlier than changes appeared in the live or the total cell number. Uptake patterns of the two tracers resembled that of 3H-thymidine, an often-used proliferation marker. We established fluorescent mesothelioma cell lines keeping an ability to form heterotpoic/orthotopic tumor mass, where tumor growth can be monitored by fluorescent intensity. In vitro study demonstrated fluorescent intensity to be a tumor size marker, whereas uptake of the PET probes to be a marker of immediate changes in tumor metabolism. |
|||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Joint Molecular Imaging Conference | |||||
| 発表年月日 | ||||||
| 日付 | 2007-09-11 | |||||
| 日付タイプ | Issued | |||||
