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Application of assisted reproductive technologies (ARTs) for radiobiological research

https://repo.qst.go.jp/records/69066
https://repo.qst.go.jp/records/69066
eafb26c4-c7e8-40a1-a72c-0c5a92984df7
Item type 会議発表用資料 / Presentation(1)
公開日 2007-07-30
タイトル
タイトル Application of assisted reproductive technologies (ARTs) for radiobiological research
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_c94f
資源タイプ conference object
アクセス権
アクセス権 metadata only access
アクセス権URI http://purl.org/coar/access_right/c_14cb
著者 Kito, Seiji

× Kito, Seiji

WEKO 677841

Kito, Seiji

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Oota, Yuki

× Oota, Yuki

WEKO 677842

Oota, Yuki

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Kaneko, Yumiko

× Kaneko, Yumiko

WEKO 677843

Kaneko, Yumiko

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Yano, Hiroko

× Yano, Hiroko

WEKO 677844

Yano, Hiroko

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Shiomi, Tadahiro

× Shiomi, Tadahiro

WEKO 677845

Shiomi, Tadahiro

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Shiomi, Naoko

× Shiomi, Naoko

WEKO 677846

Shiomi, Naoko

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Shimada, Yoshiya

× Shimada, Yoshiya

WEKO 677847

Shimada, Yoshiya

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Sakai, Kazuo

× Sakai, Kazuo

WEKO 677848

Sakai, Kazuo

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鬼頭 靖司

× 鬼頭 靖司

WEKO 677849

en 鬼頭 靖司

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太田 有紀

× 太田 有紀

WEKO 677850

en 太田 有紀

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金子 由美子

× 金子 由美子

WEKO 677851

en 金子 由美子

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矢野 浩子

× 矢野 浩子

WEKO 677852

en 矢野 浩子

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塩見 忠博

× 塩見 忠博

WEKO 677853

en 塩見 忠博

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塩見 尚子

× 塩見 尚子

WEKO 677854

en 塩見 尚子

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島田 義也

× 島田 義也

WEKO 677855

en 島田 義也

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酒井 一夫

× 酒井 一夫

WEKO 677856

en 酒井 一夫

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抄録
内容記述タイプ Abstract
内容記述 In radiation biology, experiments using animals are inevitable for understanding radiation effects at whole body level. Especially, use of genetically modified (GM) mice in radiation biology has been explosively increased in the last two decades, and an efficient system for maintenance of these animals has become a crucial issue in many radiobiological research institutes. In the National Institute of Radiological Sciences, the Advanced Animal Research Section has been collaborating with many research groups to facilitate whole animal experiments by application of assisted reproductive technologies (ARTs). The ARTs applicable to radiation research includes (1) production of new radiosensitve GM animals, (2) short and long term cryopreservation of embryos, (3) synchronous production of large number of animals, (4) domestic and international shipping of animals by way of cryopreserved embryos, and (5) cleaning of animals infected with various pathogens. An example of practical application of ARTs is simultaneous production of large animal colony by in vitro fertilization-embryo transfer (IVF-ET) system. This technique fits well for reproduction of GM mice, such as gpt-delta transgenic mice and aprt (adenine phosphorribosyltransferase) heterozygous mice used frequently for investigating radiation-induces mutagenesis in various tissues. Because of infertility derived from behavioral defect in aprt deficient homozygous (-/-) animals, they have to be bred in the colony of the heterozygous (+/-) and wild type (+/+) parents. However, maintenance of +/- animals requires laborious maintenance of both +/- and +/+ mice followed by genotyping. By application of IVF-ET to +/+ females and only two -/- males, we successfully obtained large stock of +/- aprt embryos (>500). These embryos were cryopreserved and, upon request, predetermined number of animals can be supplied without genotyping, resulting in maintenance of animals less expensively, less laboriously and more efficiently than conventional methods. Although we found some limitations in ARTs for practical application, we are currently making effort to improve our ARTs further for radiation research.
会議概要(会議名, 開催地, 会期, 主催者等)
内容記述タイプ Other
内容記述 13th International Congress of Radiation Research
発表年月日
日付 2007-07-12
日付タイプ Issued
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