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アイテム
TNF-mediated cell-death signaling pathway and extracellular matrix pathway are activated by concurrent use of cisplatin with radiotherapy in sequential biopsy specimens from patients with cervical cancer.
https://repo.qst.go.jp/records/69022
https://repo.qst.go.jp/records/69022c119bb57-d460-4ccd-ab73-59a52f2d30de
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-07-17 | |||||
| タイトル | ||||||
| タイトル | TNF-mediated cell-death signaling pathway and extracellular matrix pathway are activated by concurrent use of cisplatin with radiotherapy in sequential biopsy specimens from patients with cervical cancer. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Iwakawa, Mayumi
× Iwakawa, Mayumi× Ohno, Tatsuya× Imadome, Kaori× Nakawatari, Miyako× Sakai, Minako× Moritake, Takashi× Nakamura, Etsuko× Tamaki, Tomoaki× Katoh, Shingo× Tsujii, Hirohiko× Imai, Takashi× 岩川 眞由美× 大野 達也× 今留 香織× 中渡 美也子× 酒井 美奈子× 盛武 敬× 田巻 倫明× 加藤 眞吾× 辻井 博彦× 今井 高志 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Objective: To identify changes in gene expression related to the concurrent use of platinum compounds with radiotherapy, in the treatment of cervical cancer. Patients and Methods: Biopsy specimens were obtained from 53 patients with the uterine carcinoma, before and during fractionated radiotherapy. Twenty-five patients were treated with radiotherapy (RT) alone, while 28 received the same radiotherapy plus concomitant chemotherapy with cisplatin (CRT). Changes in gene expression induced by treatment were investigated using CodeLink single-color oligo-microarrays consisting of 44K human sequences. Paraffin-embedded samples were used to examine apoptosis and the expression of protein related with treatment-responsive genes. Changes in mRNA expression were assessed for these genes by real-time reverse transcriptase-polymerase chain reaction. Results: We found several tens of genes, including CDKN1A, BAX, TNFSF8, RRM2B, and FGF2, responded to CRT using microarray analysis, and suggested CRT activated TNF-mediated cell death pathway and extra-cellular matrix pathway related with cell death pathway. Apoptotic cells were significantly increased in both groups. In immunohistochemical study, CRT significantly increased the numbers of cases with diffusely distributed CDKN1A-positive cells (P < 0.002). Protein expression of ICAD was recognized weakly in nucleus of tumor cells in pre-treatment samples and decreased significantly after CRT (P < 0.05). BAK1 increased its intensity in half of cases at mid-treatment. The expression changes of FGF2 were revealed to be significantly different between good responders and poor responders (P < 0.05). Conclusions: CRT produced a homogenous pattern of changes in expression of known radiation-responsive genes. Our data suggest that concurrent use of cisplatin produced a radiosensitizing effect in most of these cervical cancer patients. Microarray analysis appeared a useful tool to get a comprehensive overview of the changes in gene expression after irradiation. In addition, transcriptional profiling of sequential biopsies after irradiation would improve our understanding of the biological effectiveness of radiotherapy, and provide information on potential targets for adjuvant therapy. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The 13th International Congress of Radiation Research | |||||
| 発表年月日 | ||||||
| 日付 | 2007-07-12 | |||||
| 日付タイプ | Issued | |||||
