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Comparative studies on spontaneous and ionizing radiation induced mutagenesis between somatic and male germ cells
https://repo.qst.go.jp/records/69019
https://repo.qst.go.jp/records/6901972a10314-a370-428c-8628-018a3656348e
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-07-17 | |||||
| タイトル | ||||||
| タイトル | Comparative studies on spontaneous and ionizing radiation induced mutagenesis between somatic and male germ cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Shiomi, Naoko
× Shiomi, Naoko× Noshiro, Katsuko× Kito, Seiji× Masumura, Kenichi× Nohmi, Takehiko× Shiomi, Tadahiro× 塩見 尚子× 野代 勝子× 鬼頭 靖司× 増村 健一× 能美 健彦× 塩見 忠博 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We have carried out mutation assays in germ cells from the transgenic mice after irradiation of various doses of ionizing radiation at the post-meiotic spermatid stage, to learn the difference in sensitivity to ionizing radiation in mutation induction among somatic cells and male germ cells (pre-meiotic spermatogonial stem cell and post-meiotic spermatid stage). The transgenic mice used for the assay are the gpt-delta mouse strain, which carries about 80 copies of the bacterial gpt gene per cell as targets for mutagenesis. To measure the induced mutation frequencies in male germ cells (spermatid stage), sperms were extracted at the 14th day after irradiation of 2.5 or 5 Gy of X rays, corresponding to the spermatid stage at the time of treatment. The spontaneous gpt gene mutation frequency in male germ cells was 0.36 x 10-5. The mutation frequencies in male germ cells irradiated with 2.5 or 5 Gy of X rays at the spermatid stage were 0.60 or 1.03 x 10-5, respectively. The induced mutation frequencies in male germ cells irradiated at the spermatid stage were nearly the same as those irradiated at the spermatogonial stem cell stage (0.53 or 1.05 x 10-5 for 2.5 or 5 Gy of X-ray-treatment, respectively), and about three to four times lower than those in somatic cells (2.43 or 3.46 x 10-5 for 2.5 or 5 Gy of X-ray-treatment, respectively). This difference between somatic and male germ cells in the mutation frequency would be mainly due to the high base excision repair activity in male germ cells. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The 13th International Congress of Radiation Research | |||||
| 発表年月日 | ||||||
| 日付 | 2007-07-12 | |||||
| 日付タイプ | Issued | |||||
