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Human RAD18 is involved in single-strand break repair independent of PCNA monoubiquitination
https://repo.qst.go.jp/records/69018
https://repo.qst.go.jp/records/690187301200d-9383-44a7-8553-66e6aaeabbe0
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-07-17 | |||||
| タイトル | ||||||
| タイトル | Human RAD18 is involved in single-strand break repair independent of PCNA monoubiquitination | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Shiomi, Tadahiro
× Shiomi, Tadahiro× Shiomi, Naoko× Mori, Masahiko× Tsuji, Hideo× Imai, Takashi× Inoue, Hirokazu× Tateishi, Satoshi× Yamaizumi, Masaru× 塩見 忠博× 塩見 尚子× 森 雅彦× 辻 秀雄× 今井 高志 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Proliferating cell nuclear antigen (PCNA) is monoubiquitinated in a RAD18/RAD6-dependent manner at stalled replication forks caused by DNA lesions. PCNA monoubiquitination mediates a polymerase switch from replicative to translesion polymerase to continue replication. Therefore, RAD18-deficient cells become sensitive to various DNA damaging agents. However, PCNA was not monoubiquitinated after X-irradiation in human cell line HCT116, although it's RAD18 deficient mutant was sensitive to X rays and defective in repair of X ray-induced chromosome aberrations.The mutant cells were hypersensitive to topoisomerase I inhibitor camptothecin that generated single strand breaks (SSBs) but not to topoisomerase II inhibitor etoposide that generated double strand breaks. Thus, human RAD18 is required for the repair of chromosomal SSBs and PCNA monoubiquitination is not necessary for the repair process. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The 13th International Congress of Radiation Research | |||||
| 発表年月日 | ||||||
| 日付 | 2007-07-12 | |||||
| 日付タイプ | Issued | |||||
