WEKO3
アイテム
Analysis of crosstalk between low-dose radiation-induced signaling and insulin signaling in human breast cancer cells
https://repo.qst.go.jp/records/69015
https://repo.qst.go.jp/records/6901573b80a0a-c4ce-4fee-852e-413410bb71c1
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-07-17 | |||||
| タイトル | ||||||
| タイトル | Analysis of crosstalk between low-dose radiation-induced signaling and insulin signaling in human breast cancer cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Nakajima, Tetsuo
× Nakajima, Tetsuo× et.al× 中島 徹夫 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Various cellular regulatory factors like growth hormones are considered to mediate cell proliferation and senescence in cells exposed to radiation, leading to cellular dysfunction and carcinogenesis. We have previously reported that, in the presence of insulin, MCF7 cells (human breast cancer cells) appear to be sensitive to low-dose irradiation (0.25 Gy) in MTS assays, which is based on measuring the total viable cell number. If how insulin modifies radiation-induced cell survival signaling is clarified in the cells, it is thought to be a unique model, to demonstrate signal cascades induced by low-dose irradiation. In this study, insulin-mediated sensitization of MCF7 cells was characterized by measuring cell survival using the MTS assay and colony-forming assays. Five hours after seeding, it was observed that insulin reduced cell survival in MTS assay. Using colony-forming assays, however, no change in the cell survival was observed. These results suggest that insulin induces growth inhibition in the cells after low-dose irradiation. In addition, it was observed that insulin also sensitizes to 0.25 Gy irradiation, 24 h after seeding, when cells have been fully attached to dishes and spread. This result implies that cell-cell contact and cell spreading have no effect on the insulin mediation. Since phosphatidylinositol 3 (PI3)-kinase-related cascades are known to participate in the insulin-related cascades, effects of a PI3 kinase inhibitor on the insulin mediation were evaluated. LY294002, one of PI3-kinase inhibitors sensitized to irradiation with 0.25 Gy in the absence of insulin, similarly to the insulin mediation. These results suggest that PI3-kinase works to maintain cell growth after low-dose-irradiation, and the function is possibly inhibited in the presence of insulin. To confirm the PI3-kinase participation and explore involvement of other cascades such as protein kinase C signaling in it, results of experiments using siRNA and microarray assays will be also discussed. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 国際放射線研究会議 | |||||
| 発表年月日 | ||||||
| 日付 | 2007-07-12 | |||||
| 日付タイプ | Issued | |||||
