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Molecular cloning and structural and functional characterization of new hexose transporter from cryptococcus neoformans.
https://repo.qst.go.jp/records/68758
https://repo.qst.go.jp/records/6875887e34517-bfe4-4b06-b91a-85ae8a1b1b65
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2006-11-13 | |||||
| タイトル | ||||||
| タイトル | Molecular cloning and structural and functional characterization of new hexose transporter from cryptococcus neoformans. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Chikamori, Minoru
× Chikamori, Minoru× Fukushima, Kazutaka× 近森 穣 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The genomes of C. neoformans strains have been or are currently being sequenced. Virulence is a complex phenotype and the exact nature of virulence can be difficult to elucidate, nevertheless, a polysaccharide capsule, expression of the laccase CNLAC1, and secretion of a phospholipase as virulence factors have been well documented. In the search for gene(s) involved in resistance to copper ion toxicity in C. neoformans, we identified a new hexose transporter (Hxt) gene, HXT1. Hxt1 consists of 520 amino acids and functions to transport hexoses such as glucose. With the use of the MOTIF program, Hxt1 was found to possess two sugar-transport motifs. By hydropathy analysis, 10 transmembrane domains were predicted. Each predicted transmembrane domain consisted of 22 amino acid residues, with the exception of domains II and VII, which consisted of 21 and 18 amino acid residues, respectively. Although Hxt1 conferred copper resistance to S. cerevisiae, disruption of the HXT1 gene showed that Hxt1 is not necessary for copper resistance. The growth rate of the hxt1 mutant strain was not different from that of the wild-type strain every 2 hr for 10 hr. In virulence tests, the hxt1 mutant strain showed 12% less phenoloxidase activity than the wild-type strain, and no difference in the ability to form melanin was identified. In addition, the hxt1 mutant strain showed virulence similar to that of the wild-type strain in experiments with Caenorhabditis elegans. However, the hxt1 mutant strain generated larger capsules than those of the wild-type strain. Thus, Hxt1 appears to be involved in capsule formation. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 16th Congress of the International Society for Human and Anima l Mycology | |||||
| 発表年月日 | ||||||
| 日付 | 2006-06-29 | |||||
| 日付タイプ | Issued | |||||
