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Effects of radiation on RNA interference in human breast cancer cells
https://repo.qst.go.jp/records/68546
https://repo.qst.go.jp/records/6854689a7fa42-cb32-44d0-9bda-cc0b903d9d09
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2006-06-28 | |||||
| タイトル | ||||||
| タイトル | Effects of radiation on RNA interference in human breast cancer cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Nakajima, Tetsuo
× Nakajima, Tetsuo× et.al× 中島 徹夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Small interfering RNAs(siRNAs) are substrates for the RNA-induced silencing complex(RISC). A lot of efforts have focused on the RNA interference(RNAi) using siRNAs as a tool to knock down the expression of a target protein, although all of the processes of RNAi has not been clarified yet. It is a potential therapeutic tool to be applied not only in gene therapy but also in other therapies in combination with gene therapy. In radiation therapy field, particularly, in fractionated radiation therapy and cures of patients in accidental radiation exposure, treatment with siRNA after irradiation of tissues and cells is expected to be performed in its application. However its efficacy remains unknown. Radiation affects on many biological functions. Because the siRNA function is dependent on biological regulations supported by RISC in cells, we evaluated effects of radiation on siRNA function. In human breast cancer cells(MCF7), siRNA against PKC delta down-regulated PKC delta expression 24 h after treatment. Irradiation with 5Gy inhibited the down-regulation. In the case of the siRNA against PKC alpha as well as PKC delta, radiation also inhibited the down-regulation by the siRNA. Though effects of radiation on transfection efficiency by lipofection was also assessed in the process of siRNA functioning, radiation had no effect on the transfection efficiency. These results suggest that radiation inhibits regulations of siRNA function for down regulation of targeted proteins after the internalization. This approach using radiation might lead to understanding processes of RNAi in details. The mechanism of radiation-induced inhibition of siRNA function will be discussed with the comparative data using antisense oligonucleotides. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第２０回 IUBMB国際生化学・分子生物学会議 | |||||
| 発表年月日 | ||||||
| 日付 | 2006-06-23 | |||||
| 日付タイプ | Issued | |||||
