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Drastic induction of heme oxygenase-1 expression by several caffeic acid derivatives revealed by quantitative real-time RT-PCR
https://repo.qst.go.jp/records/68545
https://repo.qst.go.jp/records/685452fd469c7-5da3-4457-8090-71ff1166b5fc
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2006-06-27 | |||||
| タイトル | ||||||
| タイトル | Drastic induction of heme oxygenase-1 expression by several caffeic acid derivatives revealed by quantitative real-time RT-PCR | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Suzuki, Keiko
× Suzuki, Keiko× Tanaka, Izumi× Nakanishi, Ikuo× Kurematsu, Ayako× Yakumaru, Haruko× Ikota, Nobuo× Ishihara, Hiroshi× 鈴木 桂子× 田中 泉× 中西 郁夫× 槫松 文子× 薬丸 晴子× 伊古田 暢夫× 石原 弘 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Cytoprotective activities of polyphenols have been reported occasionally. Among the polyphenols, caffeic acid esters such as caffeic acid phenethyl ester and chlorogenic acid are contained in propolis, vegetables and coffee. In this study, we compared the efficacy of polyphenols on the activation level of heme oxygenase-1 (HO-1) gene, which is involved in the cellular protection system, in RAW264.7 cells using quantitative real-time RT-PCR. The quantitative study revealed that there is a variety in the activation level of HO-1 gene by the chemicals. Caffeic acid phenethyl ester (CAPE) and caffeic acid ethyl ester (CAEE) at the final concentration of 2 microM drastically activated the HO-1 gene 39.2-fold and 20.1-fold, respectively. Curcumin, structurally related with caffeic acid and an element of turmeric, induced HO-1 gene as 5.8-fold. In contrast, chlorogenic acid, another caffeic acid ester, induced HO-1 as a weak level (1.3-fold) at the same concentration. Higher concentrations were necessary for the activation by antioxidants such as cysteamine and resveratrol, and an electrophile diethyl maleate. When the content of HO-1 protein in cell lysates was assayed by enzyme immunoassay, it was confirmed that HO-1 protein accumulated remarkably in the CAPE-treated cells at 4 and 8 h. Although the inducible activities of CAPE and chlorogenic acid were distinctly different, they showed similar reductive capacities when determined by a cyclic voltammetry spectroscopy. These results show that the drastic activation of HO-1 gene by CAPE and CAEE is dependent upon their chemical structures, rather than the reductive activity of polyphenols, possibly reflecting the difference among the physiological effect of nutritional elements. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress of Biochemistry and Molecular Biology | |||||
| 発表年月日 | ||||||
| 日付 | 2006-06-23 | |||||
| 日付タイプ | Issued | |||||
