WEKO3
アイテム
Quantification of [11C]verapamil transfer for evaluation of P-glycoprotein function at blood-brain barrier by Positron Emission Tomography
https://repo.qst.go.jp/records/68436
https://repo.qst.go.jp/records/68436ac2b0f02-97a0-4bd1-9a03-7ed3f521c72d
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2006-06-15 | |||||
| タイトル | ||||||
| タイトル | Quantification of [11C]verapamil transfer for evaluation of P-glycoprotein function at blood-brain barrier by Positron Emission Tomography | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Ikoma, Youko
× Ikoma, Youko× Takano, Akihiro× Ito, Hiroshi× Morimoto, Takuya× Arakawa, Ryosuke× Seki, Chie× Suzuki, Kazutoshi× Suhara, Tetsuya× 生駒 洋子× 高野 晶寛× 伊藤 浩× 森本 卓哉× 荒川 亮介× 関 千江× 鈴木 和年× 須原 哲也 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Objectives: P-glycoprotein (P-gp) is found at cell membranes of various organs, also at the blood-brain barrier (BBB), and plays a role of efflux pump hampering the invasion of toxic compounds into the cells. P-gp in the BBB has been found to be associated with several neurological disorders. [11C]verapamil has been used for the in vivo imaging of P-gp function in the BBB by positron emission tomography. However, in human studies, the metabolites in plasma complicate the quantitative analysis. In this study, we developed quantification methods of [11C]verapamil transfer from blood to the brain in human data and simulated data. Methods: In the human studies of normal volunteers, the transfer rate constant from plasma to the brain, K1, and that from the brain to plasma, k2, were estimated by nonlinear least squares (NLS) with a two-input compartment model taking account of the permeation of metabolite in plasma to the brain, and with one-input compartment model by using only 15 min data in which there was little metabolites in plasma. To avoid the measurement of metabolites in plasma, K1 was also estimated by simple graphical analysis method of an integration plot that uses only early time data (0-3min after injection), and estimated parameters were compared with that obtained by NLS methods. In the simulation study, the reliability of estimated parameters for NLS with compartment model and graphical analysis method were investigated for various values of K1 and k2, and time ranges used in parameter estimation. Results: [11C]verapamil in plasma gradually converted to its metabolites, and about 50% of the radioactivity in the plasma was associated with [11C]verapamil metabolites at 30 min after intravenous administration. Although K1 estimated from the graphical analysis method was slightly smaller than NLS methods, there was a strong correlation between the K1 values obtained by graphical analysis and that by NLS methods. In the simulated data with various K1 and k2 values, the differences between true and estimated K1 of graphical analysis method were 5.3 - 25.9% and coefficient of variation (%COV) of K1 estimates of graphical analysis method were 4.4 - 7.9% at 3% noise level. Conclusion: The transfer of [11C]verapamil from plasma to the brain was able to be quantitatively estimated by graphical analysis method without measurement of metabolites in plasma, since BBB permeability of the metabolites in plasma might not be considered during the initial few minutes after injection of [11C]verapamil. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Society of Nuclear Medicine 53rd Annual Meeting | |||||
| 発表年月日 | ||||||
| 日付 | 2006-06-06 | |||||
| 日付タイプ | Issued | |||||
