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Interaction of high-LET heavy ion irradiation and etoposide on two cell lines with different radiosensitivities and different p53 status in vitro
https://repo.qst.go.jp/records/68271
https://repo.qst.go.jp/records/682715fba2abd-bae9-4e57-9fbc-969fe9b379e6
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2005-12-01 | |||||
| タイトル | ||||||
| タイトル | Interaction of high-LET heavy ion irradiation and etoposide on two cell lines with different radiosensitivities and different p53 status in vitro | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Takahashi, Takeo
× Takahashi, Takeo× Hasegawa, Masatoshi× Fukawa, Takeshi× Furuta, Masaya× Sakurai, Hideyuki× Furusawa, Yoshiya× Ando, Koichi× Nakano, Takashi× 高橋 健夫× 長谷川 正俊× 扶川 武志× 古田 雅也× 櫻井 英幸× 古澤 佳也× 安藤 興一× 中野 隆史 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Background: To investigate the differences between two rat yolk sac tumor cell lines with different radiosensitivities in sensitivity to high-LET heavy ion beam and in sensitizing effect of etoposide (DNA topoisomerase II inhibitor) in combination with heavy ion beam. Material and methods: NMT-1 (wild-type p53 cell) is a parent radiosensitive cell line and NMT-1R (mutant-type p53 cell) is a variant radioresistant cell line. Heavy ion (carbon ion) was accelerated to 290 MeV/u by a heavy-ion medical accerelator in Chiba at National Institute of Radiological Sciences. The dose average LET value in the samples was 80 keV/um. The effects of carbon ion irradiation were assessed by clonogenic assay. The concentration of etoposide required to reduce colony formation by 50% at 1-hour treatment (IC50 of etoposide) was selected for heavy ion irradiation pretreatment for each cell line. The RBE (relative biological effect) of the carbon ion beams to X-rays was calculated for D10 (10% survival dose). Results: There was no significant difference between NMT-1 cells and NMT-1R cells in sensitivity to high-LET heavy ion irradiation (LET;80keV/um) and no shoulder in dose-response curve. The RBE was 1.41 for NMT-1 and 2.16 for NMT-1R, respectively. The RBE of carbon beam was larger in mutant-type p53 cells than in wild-type p53 cells. Etoposide showed a supra-additive effect in combination with carbon beam irradiation in NMT-1R cells. Etoposide potentiation in NMT-1R cells was manifested by the decrease in the slope of the radiation dose-response curve. However, there was no enhancement effect in radiosensitive NMT-1 cells. Conclusions: Our findings suggested that high-LET radiotherapy is expected to be effective for patients carrying radioresistant tumors and mutated p53 tumor cells. Etoposide might be effective for radioresistant tumors in combination with heavy ion beam irradiation. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | ECCO 13 | |||||
| 発表年月日 | ||||||
| 日付 | 2005-11-03 | |||||
| 日付タイプ | Issued | |||||
