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Low-Dose Radiation Response of The P21 WAF/CIP1 Gene Promoter Transduced by Adeno-Associated Virus Vector
https://repo.qst.go.jp/records/68075
https://repo.qst.go.jp/records/68075fd290fab-ed03-46f7-81e8-9e20b4bf1dd6
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2005-06-01 | |||||
| タイトル | ||||||
| タイトル | Low-Dose Radiation Response of The P21 WAF/CIP1 Gene Promoter Transduced by Adeno-Associated Virus Vector | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Nenoi, Mitsuru
× Nenoi, Mitsuru× Daino, Kazuhiro× Numata, Sachiko× et.al× 根井 充× 臺野 和広× 沼田 幸子 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | For a cancer gene therapy, control of anti-tumoric transgene expression in radiation field by use of ionizing radiation-inducible promoters is one of the approaches for the tumor-specific gene delivery. The most widely utilized promoter for this purpose is that of the early growth response 1 gene (Egr-1). However induction of the Egr-1 promoter requires a high dose of radiation, and physical burden for the patients imposed by a substantial dose of radiation is inavoidable. Although tumor suppressor protein p53 is induced by low doses (<1 Gy) of radiation, there have been only a few reports indicating potential utilization of p53-target gene promoter, such as that of the p21 gene. Recently several evidences were reported that association of p53 with its recognition sequence of DNA is dependent on chromatin structure. We here examined whether response of the p21 gene promoter to low dose radiation is enhanced when integrated into chromosomes by use of recombinant adeno-associated virus (rAAV) vectors. And availability of the p21 gene promoter in development of low-dose radiation-inducible vector for a cancer gene therapy was investigated. METHODS: A reporter construct containing luciferase gene under the control of the p21 gene promoter was transduced into MCF-7 cells by use of rAAV vector. RESULTS: It was shown that the p21 gene promoter transduced by rAAV vectors was radiation-responsive much higher than that transiently-transfected by electroporation, and suggested that the intrinsic regulation mechanism of endogenous p21 gene promoter functioned in regulation of the transduced promoter. The viral genome was observed to be randomly integrated in the chromosomes of cells. CONCLUSIONS: It was demonstrated that p53-target gene promoter in combination with virus vectors that integrate transgenes into chromosomes of host cells is potentially feasible in development of low-dose radiation-inducible vector for gene therapy | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | ヨーロッパ遺伝子治療学会第12回年会 | |||||
| 発表年月日 | ||||||
| 日付 | 2004-11-07 | |||||
| 日付タイプ | Issued | |||||
