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Hydrogen peroxide regulates the capacity of cell motility.
https://repo.qst.go.jp/records/68011
https://repo.qst.go.jp/records/68011c4d0bbcd-0112-48e7-8133-b7cccf93021f
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2005-02-05 | |||||
| タイトル | ||||||
| タイトル | Hydrogen peroxide regulates the capacity of cell motility. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Takada, Yasunari
× Takada, Yasunari× Hachiya, Misao× Akashi, Makoto× 高田 康成× 蜂谷 みさを× 明石 真言 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Mammalian cells constitutively generate the reactive oxygen species (ROS) such as superoxide anion, hydroxyl radical, and hydrogen peroxide through normal intracellular metabolic processes.; The generation is then increased in response to physiological stimuli including cytokines or and growth factors, the generation is increased. Recent studies have shown that ROS are important second messengers. The capacity of cell motility is important for tissue repair and cell-cell interaction in normal cells. Manganese superoxide dismutase (MnSOD) is located in mitochondria and catalyses the dismutaion of superoxide anion into hydrogen peroxide. We studied the role of ROS in cell motility modulating the cellular redox status by introducing human MnSOD cDNA into human ovarian cancer cells, SK-OV-3. Introduction of MnSOD cDNA increased the level of hydrogen peroxide in SK-OV-3 cells. Treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA) induced cell motility with a concomitant increase in the level of hydrogen peroxide in these cells. Overexpression of MnSOD enhanced cell motility and generation of hydrogen peroxide induced by TPA. Treatment with TPA also induced phosphorylation of p42/44 extracellular signal-regulated kinase 1 and 2 (ERK1/2) in cells overexpressing MnSOD. However, TPA did not affect the phosphorylation of ERK1/2 in control cells. Inhibition of the ERK1/2 pathway abrogated TPA-induced cell motility in MnSOD transfectants. Diethylmaleate (DEM) depletes glutathione and acts as a pro-oxidant, leading to the accumulation of hydrogen peroxide. Treatment with DEM activated ERK1/2 and induced cell motility in both cell lines. Further study found that introduction of oncogenic Rasv13 into SK-OV-3 cells induced cell motility with a concomitant increase in the level of intracellular hydrogen peroxide. In addition, Rasv13 -induced cell motility was inhibited by microinjection of human catalase cDNA. Thus, our results suggest that hydrogen peroxide regulates the capacity of cell motility induced by TPA through a pathway requiring ERK1/2 activation. The present study also indicates that hydrogen peroxide plays an important role in cell motility of Ras-transformed cells. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 95th Annual Meeting 2004 | |||||
| 発表年月日 | ||||||
| 日付 | 2004-03-31 | |||||
| 日付タイプ | Issued | |||||
