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BMP Regulation of SOST and Osterix Expression During Embryonic Osteogenesis
https://repo.qst.go.jp/records/67906
https://repo.qst.go.jp/records/67906423e876e-86d6-4a40-94c0-80b3180f72a2
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2004-10-07 | |||||
| タイトル | ||||||
| タイトル | BMP Regulation of SOST and Osterix Expression During Embryonic Osteogenesis | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Nifuji, Akira
× Nifuji, Akira× 二藤 彰 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Sclerostin (SOST), a member of the cystine-knot superfamily, is essential for proper skeletogenesis since loss of function mutation in SOST gene results in sclerosteosis featured with massive bone growth in humans. To understand the function of SOST in developmental skeletal tissue formation, we examined SOST gene expression in embryonic osteogenesis in vitro and in vivo. During osteoblastic differentiation in primary calvarial cells, the levels of SOST expression were increased along with those of alkaline phosphatase activity and nodule formation. In situ hybridization study revealed that SOST mRNA expression was observed in osteogenic front in embryonic 16.5 day postcoitum (E16.5) embryonic calvariae and this expression persisted in the peripheral area of cranial bone in the later developmental stage (E18.5). These temporal and spacial expression patterns in vivo and in vitro were in parallel to those of osterix (Osx), which is a critical transcriptional factor for bone formation. Similar co-expression of SOST and Osx mRNA was observed when the primary osteoblastic calvarial cells were cultured in the presence of BMP2 in vitro. Moreover, endogenous expression of SOST and Osx mRNA was inhibited by infection of noggin-expressing adenovirus into the primary calvarial osteoblastic cells, suggesting that endogenous BMPs are required for these cells to express SOST and Osx mRNA. Thus, expression and regulation of SOST under the control of BMP were closely associated with those of Osx in vivo and in vitro. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | American Society for Bone and Mineral Research 26th annual meeting | |||||
| 発表年月日 | ||||||
| 日付 | 2004-10-05 | |||||
| 日付タイプ | Issued | |||||
