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Correlation between cellular radiosensitivity and DNA damage measured by the alkaline comet assay in human cancer cell lines.
https://repo.qst.go.jp/records/67652
https://repo.qst.go.jp/records/676520c2691f0-1907-41ef-aeb5-3adcafa78335
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2003-11-25 | |||||
| タイトル | ||||||
| タイトル | Correlation between cellular radiosensitivity and DNA damage measured by the alkaline comet assay in human cancer cell lines. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Gotou, Miyako
× Gotou, Miyako× Iwakawa, Mayumi× Noda, Syuuhei× Saegusa, Kumiko× Kawai, Seiko× Harada, Yoshinobu× Imai, Takashi× 中渡 美也子× 岩川 眞由美× 野田 秀平× 三枝 公美子× 川井 聖子× 原田 良信× 今井 高志 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In order to evaluate the usefulness of alkaline comet assay as a predictive assay of radiosensitivity, two parameters of comet assay and three parameters of cell survival curve were compared using 13 human cancer cell lines. Colony forming assay was performed after irradiation from 1 Gy to 6 Gy, and determined clonogenic survival parameters, such as D10, Dq and SF2. In comet assay, initial DNA damage (ID) immediatery after X-ray irradiation at dose of 8 Gy and residual damage (RD) at each 30 or 60 min after irradiation were assessed. Thirteen cell lines showed a broad spectrum in D10 value, ranging from 2.74 to 8.18, and Dq value, ranging from 0.29 to 3.42. ID varied from 1.87 to 9.09 among the cell lines, and RD varied from 0.66% to 56.51% at 30 min, and from 0% to 55.58% at 60 min. When cell lines were classified into two groups (group A and group B) according to each own D10 value, the group A, whose D10 value was more than 6, showed higher ID (5.4 + 2.3) than the group B (2.6 + 0.7), whose D10 values was less than 6 (P=0.008). There was, however, no correlation between ID and SF2. When cell lines were classified into two groups according to each own Dq value (group C and group D), the average of RD in group C, whose Dq value was more than 2, was 2.9% at 30 min. Group D whose Dq value is less than 2 was 15.6% at 30 min. The significant difference was observed between group C and group D (p= 0.049). These results suggest that the comet assay might be potential to classify a radiosensitive group according to ID and RD. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | International Comet Assay Workshop No.5 | |||||
| 発表年月日 | ||||||
| 日付 | 2003-08-31 | |||||
| 日付タイプ | Issued | |||||
