WEKO3
アイテム
TIGHT JUNCTION OF MAMMARY EPITHELIAL CELLS IS INFLUENCED BY NITRIC OXIDE
https://repo.qst.go.jp/records/67601
https://repo.qst.go.jp/records/676017dbc35ce-cb27-42cf-b685-304f1bb77e3c
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2003-11-07 | |||||
| タイトル | ||||||
| タイトル | TIGHT JUNCTION OF MAMMARY EPITHELIAL CELLS IS INFLUENCED BY NITRIC OXIDE | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
小野田, 眞
× 小野田, 眞× 小野田, 真美× 稲野, 宏志× 勝部, 孝則× 小野田 眞× 小野田 真美× 稲野 宏志× 勝部 孝則 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Aim: Nitric oxide (NO) is an essential biological molecule with a wide variety of functions in physiological and pathophysiological events. We demonstrated previously that administration of NO scavenger or NO synthase inhibitor decreased the incidence of mammary tumors of rat irradiated with gamma-ray. Thus NO might participate in the radiation-induced tumorigenesis. To elucidate the role of NO in cellular and molecular events, we examined the effect of NO on the expression and localization of tight junction-associated proteins in a mouse mammary epithelial cell line (HC11). Methods: Monolayer of confluent HC11 was incubated with either NOC5 (20 mM) or NOC18 (0.4 mM), NO donors, for 90 min or 48 hr, respectively, and used for analyses of cell-cell junctional proteins by immunochemical and immunocytochemical means. Results and Discussion: Continuous localization around the cell perimeter of occludin and ZO-1, components of tight junction, was disorganized, and descending expression of these tight-junctional proteins was revealed in HC11 treated with NOC18. On the other hand, tyrosine phosphorylation of several proteins was induced in a non-ionic detergent insoluble fraction of the cells incubated with NOC5. Furthermore, immunocytochemical observation for the phosphorylated proteins in the NOC5 treated cells showed distinct labeling at cell-cell junction sites. These results suggest that NO influences upon expression and localization of tight junctional proteins in mammary epithelial cells, and that the phosphorylation of these proteins may participate in functional modification of tight junction. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第７６回日本生化学会 | |||||
| 発表年月日 | ||||||
| 日付 | 2003-10-18 | |||||
| 日付タイプ | Issued | |||||
