WEKO3
アイテム
UV- irradiation induces oxidative damage to mitochondrial DNA primarily through hydrogen peroxide
https://repo.qst.go.jp/records/67577
https://repo.qst.go.jp/records/675773313881e-8409-4e90-b0b5-222bec9a4f03
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2003-11-05 | |||||
| タイトル | ||||||
| タイトル | UV- irradiation induces oxidative damage to mitochondrial DNA primarily through hydrogen peroxide | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Takai, Daisaku
× Takai, Daisaku× Hirama, Toshiyasu× Hachiya, Misao× Akashi, Makoto× 高井 大策× 平間 敏靖× 蜂谷 みさを× 明石 真言 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Mitochondria are cell organella observed in eukaryotic cells and they play important roles in various basic biological phenomena such as apoptosis as well as production of energy via oxidative phosphorylation. Mitochondria have their own genome, mitochondrial DNA (mtDNA), in the matrix. In this decade, studies have found that mutations of mtDNA occur in various types of diseases and these mutations may result from oxidative damages by reactive oxygen species (ROS) generated in mitochondria. Furthermore, cytotoxic stress such as inadequate accumulation of ROS triggers pathways of apoptosis in mitochondria; mitochondria release cytochrome c into cytoplasma. Thus, mitochondria are a central regulatory element in stress-induced events. UV-irradiation activates the generation of ROS in cells, leading to consequent mutations in mtDNA. However, eukaryotes have the self-defense mechanisms against the adverse effects exerted by ROS. Manganese superoxide dismutase (MnSOD) and catalase (CAT) are thought to be involved in the mechanisms and to convert ROS to less toxic substances; MnSOD catalyses O2- to H2O2 and CAT converts H2O2 to water. We studied further roles of ROS in mtDNA damage by ROS in UV-irradiation. We introduced a cDNA of MnSOD and/or CAT into the human hepatoma cell line, SK-HEP1. Analyses using HPLC showed that UV-irradiation increased the accumulation of 8-hydroxy-2'-deoxyguanosine (8OHdG), a common marker for oxidatively damaged mtDNA, in these cells; a significantly increased level of 8OHdG was observed in all these cell lines. UVirradiation induced the accumulation of 8OHdG at higher level in MnSOD transfectants than in the control and CAT- or MnSOD/CAT- transfectants. Upon UV-irradiation, on the other hand, the accumulation of 8OHdG was reduced in cells overexpressing CAT when compared to that in the control cells. The accumulated levels of 8OHdG were in parallel with those of H2O2. Our results suggest that H2O2 play a key role in mtDNA damage in UV-irradatied cells. Our results also indicate that increased activity of MnSOD may cause a toxic effect on mtDNA by UV-irradiation, if the H2O2 cannot be converted. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Apoptosis2003 | |||||
| 発表年月日 | ||||||
| 日付 | 2003-02-01 | |||||
| 日付タイプ | Issued | |||||
