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Radiation-induced germ-line mutations detected by a direct comparison of parents and children DNA sequences containing SNPs
https://repo.qst.go.jp/records/67442
https://repo.qst.go.jp/records/67442ea2464e7-96d3-4269-acb9-9b5eaccad8e3
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2003-08-27 | |||||
| タイトル | ||||||
| タイトル | Radiation-induced germ-line mutations detected by a direct comparison of parents and children DNA sequences containing SNPs | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Morimyo, Mitsuoki
× Morimyo, Mitsuoki× Hongou, Etsuko× Higashi, Tomoyasu× K, Go× Matsumoto, Izumi× Okamoto, Masanori× Kawano, Akihiro× Tsuji, Satsuki× 森明 充興× 本郷 悦子× 東 智康× 呉 健羽× 松本 いづみ× 岡本 正則× 河野 明広× 辻 さつき |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Germ-line mutation is detected in mice but not in humans. To estimate genetic risk of humans, a new approach to extrapolate from animal data to humans or to directly detect radiation-induced mutations in man is expected. We have developed a new method to detect germ-line mutations by directly comparing DNA sequences of parents and children. The nucleotide sequences among mouse strains are almost identical except SNP markers that are detected at 1/1000 frequency. When gamma-irradiated male mice are mated with female mice, heterogeneous nucleotide sequences induced in children DNA are a candidate of mutation, whose assignment can be done by SNP analysis. This system can easily detect all types of mutations such as transition, transversion, frameshift and deletion induced by radiation and can be applied to humans having genetically heterogeneous nucleotide sequences and many SNP markers. C3H male mice of 8 weeks of gestation were irradiated with gamma rays of 3 and 1 Gy and after 3 weeks, they were mated with the same aged C57BL female mice. After 3 weeks breeding, DNA was extracted from parents and children mice. The nucleotide sequences of 150 STS markers containing 300-900 bp and SNPs of parents and children DNA were determined by a direct sequencing; amplification of STS markers by Taq DNA polymerase, purification of PCR products, and DNA sequencing with a dye-terminator method. At each radiation dose, a total amount of 5 Mb DNA sequences were examined to detect radiation-induced mutations. We could find 6 deletions in 3 Gy irradiated mice but not in 1 Gy and control mice. The mutation frequency was about 4.0 x 10-7/bp/Gy or 1.6 x 10-4/locus/Gy, and suggested the non-linear increase of mutation rate with dose. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第１２回国際放射線研究会議 | |||||
| 発表年月日 | ||||||
| 日付 | 2003-08-22 | |||||
| 日付タイプ | Issued | |||||
