WEKO3
アイテム
A new experimental procedure for evaluating the biological effect of radon
https://repo.qst.go.jp/records/67435
https://repo.qst.go.jp/records/67435882d4f33-6c2c-4165-b1a3-8d1dd45a20c2
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2003-08-26 | |||||
| タイトル | ||||||
| タイトル | A new experimental procedure for evaluating the biological effect of radon | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yamauchi, Masatake
× Yamauchi, Masatake× Azuma, Hiroto× Kanai, Reiko× Yamada, Yuji× Fukutsu, Kumiko× Hatada, Takako× 山内 正剛× 東 裕人× 内山 禮子× 山田 裕司× 福津 久美子× 畠田 貴子 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Inhalation of radon and its short-lived progeny gives a considerable amount of radiation dose to the general public. The dosimetry for the radon risk estimation has been attempted by comparing the cancer incidence between different populations including underground miners but not yet suffi ciently accurate to provide quantitative estimates of risks of the radon inhalation. To understand the molecular basis for the lifetime cancer risk caused by the internally deposited radionuclides, especially the alpha particle- emitting nuclides, we planned to establish a simple experimental system using mammary cultured cells. We used mouse FM3A cells, derived from mammary grand tumor cells, that grow on soft agar plates with the plating effi ciency of >90%. Since the cells can grow while being exposed to the atmosphere, we thought it could be an ideal model system to examine the biological effect of radon on epithelial cells. As a preliminary experiment, exponentially growing FM3A cells were exposed to X ray (0, 0.3, 1, 3, and 10Gy), and plated on the medium containing soft-agar. Colonies formed on the soft-agar plates were picked up, and DNA samples extracted from the cells were analyzed at the molecular level by using the direct DNA sequencing method, as well as the Southern blot hybridization technique. For direct DNA sequencing, adenine phosphoribosyl transferase gene was chosen as a typical case of non-essential genomic gene and cytidine triphosphate synthetase as an essential one. For the Southern blot analysis, Pc-1 (Ms6-hm) was chosen as a hypervariable tandem repeat sequence, and its genomic instability was examined. We will explain the usefulness of this new experimental procedure for the evaluation of the biological effect caused by radon and its short-lived progeny. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第１２回国際放射線研究会議 | |||||
| 発表年月日 | ||||||
| 日付 | 2003-08-22 | |||||
| 日付タイプ | Issued | |||||
