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Studies on a role of XRCC4 in human cells
https://repo.qst.go.jp/records/67424
https://repo.qst.go.jp/records/67424eb372b90-9c83-4cc9-95f9-5369cc94d246
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2003-08-25 | |||||
| タイトル | ||||||
| タイトル | Studies on a role of XRCC4 in human cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Mori, Masahiko
× Mori, Masahiko× Itsukaichi, Hiromi× Kanda, Reiko× Nakamura, Atsuko× Shiomi, Naoko× Aizawa, Shirou× Shiomi, Tadahiro× 森 雅彦× 五日市 ひろみ× 神田 玲子× 中村 篤子× 塩見 尚子× 相澤 志郎× 塩見 忠博 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Ionizing radiation produces a variety of lesions in DNA including single-strand breaks, double strand breaks and base damage. The repair of DNA double-strand breaks in essential for the maintenance of genomic integrity. Failure to repair DNA double-strand breaks result in loss of genetic information, chromosome translocations, carcinogenesis and cell death. XRCC4 is a member of non-homologous end-joining proteins that functioned in DNA double-strand break repair in eukaryote including human. XRCC4 is a DNA ligase IV accessory factor and required for the rejoining of DNA double- strand breaks. Both XRCC4 and DNA ligase IV deficient mice have been generated. Both deficient mice are not viable because of neuronal degeneration caused by p53-induced apoptosis. Cells obtained from XRCC4 or DNA ligase IV deficient embryo are viable, but show reduced cell proliferation and hypersensitivity to ionizing radiation. To study the role of XRCC4 in human cells, we tried to inactivate XRCC4 gene by using gene targeting technology in human colon cancer cell line, HCT116. We have succeeded to disrupt both alleles of XRCC4 gene. Heterozygous (XRCC4+/-)cells showed reduced cell proliferation but normal X Ray-sensitivity, indicating haploinsufficiency in cell proliferation but not in X ray-sensitivity. Homozygous (XRCC4-/-) cells show reduced cell proliferation and increased chromosome aberrations, and are highly sensitive to X-rays. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 12th International Congress of Radiation Research | |||||
| 発表年月日 | ||||||
| 日付 | 2003-08-22 | |||||
| 日付タイプ | Issued | |||||
