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gammaH2AX foci formation in x-irradiated mammalian cell lines with different radio-sensitivities
https://repo.qst.go.jp/records/67421
https://repo.qst.go.jp/records/67421413e6600-270a-44c3-b960-6e6c933140b7
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2003-08-25 | |||||
| タイトル | ||||||
| タイトル | gammaH2AX foci formation in x-irradiated mammalian cell lines with different radio-sensitivities | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Sagara, Masashi
× Sagara, Masashi× Ban, Sadayuki× Sudou, Hitomi× Okayasu, Ryuichi× Imai, Takashi× 相良 雅史× 伴 貞幸× 須藤 仁美× 岡安 隆一× 今井 高志 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | DNA double-strand breaks (DSBs) induced by ionizing radiation represent the most serious damage in cells. Immediately after the formation of DSB, Ser-139 of histone H2AX is phosphorylated at the site of DSB. The phosphorylated H2AX (gammaH2AX) foci can be observed with an immunohistochemical staining using anti-gammaH2AX antibody. It is also known that each gammaH2AX focus corresponds to one DSB and disappears when the DSB is rejoined. Using this method, we compared the amount of unrejoined DSBs among cell lines with different radiation sensitivities after X irradiation. At 30 min following 2 Gy of X-rays, the number of foci observed is similar in all cell lines studied. However, the disappearance of foci in radioresiatant colon cancer cell lines (SW-480 and CaCo-2) was faster than that in radiosensitive ones (SW-48 and LoVo). At 12 hr after X-irradiation, few foci were observed in SW-480 and CaCo-2 cells, while many foci were remained in SW-48 and LoVo cells. Furthermore, the number of focus disappeared much slower in irradiated radiosensitive AT and 180BR cells as compared to normal human fibroblasts. Similar results were obtained when cells from radiosensitive LEC rat were compared with cells from Fisher rat. Our data demonstrate that the rejoining kinetics of DSBs measured by gammaH2AX foci formation correlates with the cellular radiosensitivities. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The 12th International Congress of Radiation Research(ICRR) | |||||
| 発表年月日 | ||||||
| 日付 | 2003-08-22 | |||||
| 日付タイプ | Issued | |||||
