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Analysis of unrejoined chromosome breakage in human fibrobrast cells exposed to low- and high-LET radiation
https://repo.qst.go.jp/records/67260
https://repo.qst.go.jp/records/672607c11d958-9fd7-4dd8-8d10-1512cca06a39
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2002-07-18 | |||||
| タイトル | ||||||
| タイトル | Analysis of unrejoined chromosome breakage in human fibrobrast cells exposed to low- and high-LET radiation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Wu, Honglu
× Wu, Honglu× Furusawa, Yoshiya× George, Kerry× Kawata, Tetsuya× A, Cucinotta Francesca× ウー ホングル× 古澤 佳也× ジョージ ケリー× 川田 哲也× クシノッタ フランク |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Reported studies of radiation induced DNA breakage have generally shown higher fractions of residual unrejoined breaks after high-LET exposures compared with low-LET exposures. This observation is supported by the argument that DNA breaks produced by high-LET radiation are more complex and, thus, less likely to be repaired. However, the dose used in these studies was generally very high. We studied unrejoined chromosome breakage by analyzing chromosome aberrations using a fluorescence in situ hybridization (FISH) technique with a combination of whole chromosome specific probes and probes for the telomere regions of all chromosomes. Confluent human fibroblast cells (AG1522) were irradiated with g rays, 490 MeV/nucleon Si, 200 MeV/nucleon Fe, or 500 MeV/nucleon Fe ions, and were incubated at 37 0C for 24 hours after exposure. A chemically induced premature chromosome condensation (PCC) technique was used to condense chromosomes mostly in the G2 phase of the cell cycle. Results showed that the frequency of unrejoined chromosome breaks was LET dependant, but the ratio of unrejoined to misrejoined breaks remained low for LET values up to 440 keV/mm. Therefore, for both low- and high-LET most chromosome breaks were either rejoined or misrejoined, when observed in the G2/M phase of the cell cycle. Unrejoined chromosome breaks associated with incomplete exchanges were also analyzed using an m-FISH technique where human chromosomes were painted in 24 different colors. As with the telomere analysis, the m-FISH analysis showed in a higher fraction of unrejoined chromosome breaks. It was also deduced that the ratio of unrejoined to misrejoined chromosome breaks, or of incomplete to complete interchromosomal exchanges is a potential biomarker for radiation quality for a wide range of LET values. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The 2nd International Workshop on Space Radiation Research | |||||
| 発表年月日 | ||||||
| 日付 | 2002-03-15 | |||||
| 日付タイプ | Issued | |||||
