WEKO3
アイテム
Development of a microscope/PET simultaneous measurement system for awake mice
https://repo.qst.go.jp/records/65498
https://repo.qst.go.jp/records/6549817b3bc86-cc4d-46a5-84da-37344bd2d01a
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-09-22 | |||||
| タイトル | ||||||
| タイトル | Development of a microscope/PET simultaneous measurement system for awake mice | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
田桑, 弘之
× 田桑, 弘之× al., et× 田桑 弘之 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Objectives: In positron emission tomography (PET), administered radiotracer in brain is mainly delivered to each tissue by cerebral blood flow (CBF) via a blood vessel. Therefore, it is very important to understand an influence of CBF on PET measurement. Moreover, functions of neurotransmission system (dopaminergic system, serotonergic system, etc.) and glucose metabolism, which can be measured by PET, are closely associated with the other brain function, including neural activation, oxygen metabolism and CBF. Thus, a simultaneous measurement of several physiological parameters is essential for the study of brain disease mechanism and the development of the approach to therapy of them in PET study. Optical imaging using CCD camera in animal study is ideally suited to measure the physiological parameters (CBF (laser speckle imaging (LSI)), neural activation (voltage sensitive dye imaging) and oxygen metabolism (flavoprotein autofluorescence imaging)), and combining this measurement with a PET dynamic scan for the quantitative analysis would be challenging. In this study, we developed the simultaneous measurement system of optical imaging and PET for awake mice. As a first step of this study, we applied this system to a simultaneous measurement of CBF with LSI and concentration of 11C-raclopride for dopamine D2 receptors with PET. Methods: The key idea is an open PET, which allows combination of another device. A prototype of a single-ring OpenPET (SROP), which can provide an accessible and observable open space, was used (Fig. 1A and 1B). Objective lens of microscope with CCD cameras (MiCAM02, Brainvision, Tokyo, Japan) was putted inside the open space of SROP. Awake animal (C57BL/6J mice, 27g) was set on a hand-made fixation apparatus in SROP. This apparatus consisted of a head holder and styrofoam ball float on a jet of air under the mice. This allows the mice to walk freely on the ball during PET scan and optical imaging. Measurements of CBF and accumulation of 11C-raclopride were performed simultaneously. CBF was measured with LSI through a chronic cranial window at brain in awake mice. In PET measurement, a dynamic scan was performed for 60 min after intravenous infusion of 11C-raclopride. Results and conclusion: Our apparatus successfully obtained CBF and radioactivity concentration of 11C-raclopride simultaneously. Accumulation of 11C-raclopride was observed in the striatum where the density of dopamine D2 receptors is high (Fig. 1C). On the other hand, accumulation of 11C-raclopride was relatively low at the cerebellum where the specific binding to receptors is negligible (Fig. 1D). Map of CBF level by LSI was shown in Fig. 1E and F. Results of both maps of CBF suggested that LSI can be stably performed during 90 minutes inside OpenPET. It was well known that anesthesia greatly affects the animal physiology, including neuronal and vascular functions. However, using our awake mice experiment system, we can ignore the effects of anesthesia on results obtained by simultaneous imaging system. We concluded that our imaging system in an awake animal model should be useful for investigating the mechanism of brain disease. |
|||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | World Molecular Imaging Congress (WMIC) 2014にて口頭発表のため。 | |||||
| 発表年月日 | ||||||
| 日付 | 2014-09-18 | |||||
| 日付タイプ | Issued | |||||
