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アイテム
Effects of Carbon Ion Beam Alone or in Combination with Chk1 Inhibitor and Gemcitabine on Putative Pancreatic Cancer Stem Cells
https://repo.qst.go.jp/records/65137
https://repo.qst.go.jp/records/651379da2d555-d692-47e1-b611-93dd89361ecc
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-09-17 | |||||
| タイトル | ||||||
| タイトル | Effects of Carbon Ion Beam Alone or in Combination with Chk1 Inhibitor and Gemcitabine on Putative Pancreatic Cancer Stem Cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Sai, Sei
× Sai, Sei× Shirai, Toshiyuki× Kamada, Tadashi× 崔 星× 白井 敏之× 鎌田 正 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Aims The aim of this study is to investigate whether a carbon ion beams alone or in combination with AZD7762 and gemcitabine has beneficial effects compared to X-ray by targeting putative human pancreatic cancer stem cells (CSCs). Materials and Methods Human pancreatic CSCs sorted from PANC1, MiaPaca2, BxPc3, and PK45 cells were treated with carbon ion or X-ray alone or in combination with Chk1 Inhibitor AZD7762 and gemcitabine, and then colony, spheroid and tumor formation assays, immunofluorescence γH2AX foci assay as well as apoptosis analysis were performed. Results The colony, spheroid formation as well as tumorigenicity assays confirmed that CD44+/CD24+ and CD44+/ESA+ cells exactly have CSC properties compared to CD44-/CD24- and CD44-/ESA- cells. CSCs were more highly enriched after X-ray or gemcitabine compared to carbon ion beam. Carbon ion beam combined with AZD7762 and/or gemcitabine significantly suppressed colony and spheroid formation compared to that of X-ray combined with AZD7762 and/or gemcitabine as well as carbon ion alone. Immunofluorescence assay showed that not only the number but also the size of γH2AX foci in CSCs were lager 24 h after carbon ion in combination with AZD7762 and gemcitabine compared to that of X-ray combined with AZD7762 and/or gemcitabine as well as carbon ion alone. The number of apoptotic CSCs 48 h after treatment with carbon ion beam in combination with AZD7762 and/or gemcitabine were significantly increased compared to that of X-ray combined with AZD7762 and/or gemcitabine as well as carbon ion alone. Conclusion Carbon ion beam combined with Chk1 inhibitor and/or gemcitabine has superior potential to kill pancreatic CSCs with unrepairable severe DNA damage and enhanced apoptosis. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | International Association of Pancreatology (IAP) and Korean Pancreatobiliary Association (KPBA) | |||||
| 発表年月日 | ||||||
| 日付 | 2013-09-07 | |||||
| 日付タイプ | Issued | |||||
