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Acceleration of tumor acetate uptake under hypoxia: A new perception of tumor imaging by acetate
https://repo.qst.go.jp/records/65111
https://repo.qst.go.jp/records/65111d4f780e3-e3fc-49fb-9400-40af1478a57e
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-08-29 | |||||
| タイトル | ||||||
| タイトル | Acceleration of tumor acetate uptake under hypoxia: A new perception of tumor imaging by acetate | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yoshii, Yukie
× Yoshii, Yukie× Furukawa, Takako× Yoshii, Hiroshi× Fujibayashi, Yasuhisa× 古川 高子× 藤林 康久 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Objectives: 11C-acetate PET is known as a useful tool for detecting various kinds of tumors. The acetate uptake in tumor cells is considered to be mediated by acetyl-CoA synthetase (Acss). On the other hand, our recent data showed that expression of Acss was up-regulated under hypoxia, which suggested that this enzyme plays important roles to control both anabolism and catabolism of acetate coordinately in tumor cells. In this study, we characterized tumor acetate uptake under hypoxia to propose a new perception of acetate tumor imaging. Methods: in vitro 14C-acetate uptake study was performed using four mouse tumor cell lines (lung carcinoma, melanoma, colon carcinoma, and mammary carcinoma) and a mouse fibroblast cell line as control. Cells were plated and incubated under normoxia for 24 h and some cells were treated by 2-6 h hypoxia, additionally. After the preincubation, medium containing 1 mu Ci 14C-acetate was added to each well of 24-well plates and respective cells were incubated for 1 h under the same condition. Then, medium was removed and cells were washed twice and lysed with 0.2N NaOH or processed by Bligh and Dyer extraction method to separate lipid- and water-soluble fractions. Radioactivity was measured by a liquid scintillation counter. The results were compared to Acss mRNA expression pattern. Results: 14C-acetate uptake was higher under hypoxia than normoxia in tumor cells, but not in normal cells. In tumor cell lines, ratio of 14C incorporation into lipid-soluble fraction to that into water-soluble fraction increased 2-3 times under hypoxia, compared to normoxia. The pattern of 14C-acetate uptake was correlated with that of Acss2 mRNA expression. Conclusions: As acetate metabolism catalyzed by Acss was up-regulated under hypoxia, incorporation of 14C-acetate increased in tumor cells. Most of the acetate taken up by tumor cells seemed to be consequently fixed into lipid-soluble fraction under hypoxia. These evidences suggest a possibility that 11C-acetate PET is visualizing hypoxic up-regulation of acetate metabolism in tumor cells. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Society of nuclear medicine 54rd Annual Meetng | |||||
| 発表年月日 | ||||||
| 日付 | 2007-06-06 | |||||
| 日付タイプ | Issued | |||||
