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Threshold number of protons for inducing adaptive response in zebrafish embryos
https://repo.qst.go.jp/records/64735
https://repo.qst.go.jp/records/64735e3b7adc7-95e9-4918-ad27-d740f88ba3dd
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-09-19 | |||||
| タイトル | ||||||
| タイトル | Threshold number of protons for inducing adaptive response in zebrafish embryos | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Choi, Viann
× Choi, Viann× Konishi, Teruaki× Oikawa, Masakazu× H., Cheng Shuk× Yu, Peter× 小西 輝昭× 及川 将一 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Radioadaptive response (RAR) refers to the biological response where an exposure of cells or animals to a low dose of radiation (priming dose) induces mechanisms that protect the cells or animals against the detrimental effects of a subsequent larger radiation exposure (challenging dose). RAR induction depends on the magnitude of the priming dose. In the present work, zebrafish embryos were used as the in vivo model. An advantage of using zebrafish embryos was that the human and zebrafish genomes shared considerable homology, including conservation of most DNA repair-related genes. The microbeam irradiation system (Single-Particle Irradiation System to Cell, acronym as SPICE) at the National Institute of Radiological Sciences (NIRS), Japan, was employed to provide the priming dose. The embryos were dechorionated at 4 h post fertilization (hpf) and irradiated at 5 hpf by microbeam protons. For each embryo, one irradiation point was arbitrary chosen, to which 5, 10, 20, 30, 40, 50, 100, 200, 300 or 500 protons each with an energy of 3.4 MeV were delivered. About 30 embryos were used for each irradiation condition. The embryos were returned back to the incubator until 10 hpf to further receive the challenging exposure, which was achieved using 2 Gy of X-ray irradiation, and then again returned to the incubator until 24 hpf for analyses. The levels of apoptosis in zebrafish embryos at 25 hpf were quantified through TUNEL assay. The results revealed that at least 200 protons or equivalently 430 muGy would be required to induce RAR in the zebrafish embryos. Our previous investigation showed that 5 protons delivered at 10 points on an embryo, or equivalently 110 muGy, would already be sufficient to induce RAR in the zebrafish embryos. Possible reasons for this difference are discussed in the paper. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | International Symposium on NATURAL RADIATION EXPOSURES and LOW DOSE RADIATION EPIDEMIOLOGICAL STUDIES 2012 | |||||
| 発表年月日 | ||||||
| 日付 | 2012-03-03 | |||||
| 日付タイプ | Issued | |||||
