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Alteration in the DNA methylation profile of irradiated cancer cell lines
https://repo.qst.go.jp/records/64586
https://repo.qst.go.jp/records/645866b76fa2a-8c29-47c4-a4e6-48f1fc8a7cf6
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-05-11 | |||||
| タイトル | ||||||
| タイトル | Alteration in the DNA methylation profile of irradiated cancer cell lines | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
石川, 顕一
× 石川, 顕一× 石川, 敦子× 荘司, 好美× 藤田, 真由美× 金井, 昭教× 飯塚, 大輔× 今井, 高志× 石川 顕一× 石川 敦子× 荘司 好美× 藤田 真由美× 飯塚 大輔× 今井 高志 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | DNA methylation is one of the epigenetic modifications that occurs in genome of mammals to ensure the proper regulation of gene expression in response to environmental stimuli and stable gene silencing. However, the regulation of gene expression by DNA methylation after genotoxic stress including irradiation remains poorly understood. We hypothesized that irradiation may elicit alterations in the methylation status of DNA and subsequently alter gene expression. In this study, using next generation sequencing technology we assessed the DNA methylation status in MIAPaCa-2 and PANC-1 cell lines (both derived from human pancreatic cancer), at 48 h after irradiation with X-ray at 4 Gy, or a carbon ion beam at 2 Gy. Methylated DNA fragments were concentrated using methyl binding protein after fragmentation of DNA to an average fragment size of 200 bp. Genome regions showing a different methylation status after irradiation were detected using MACS algorithm. Complete methylation or de-methylation of 1.6 Mb or 6.7 Mb regions in both MIAPaCa-2 and PANC-1 cell lines occurred after X-ray irradiation, and the methylation status of 1.0 Mb or 1.7 Mb regions also changed in the both cell lines after carbon ion beam irradiation. Both X-ray and carbon ion beam irradiation altered the methylation status in approximately 5 % of these regions. As expected, in approximately 300 regions, altered DNA methylation following irradiation occurred within 500 bp upstream of a possible transcriptional start site. Studies are in progress to examine the relationship between alteration of DNA methylation status and gene expression in cells following irradiation. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第34回日本分子生物学会年会(MBSJ2011) | |||||
| 発表年月日 | ||||||
| 日付 | 2011-12-16 | |||||
| 日付タイプ | Issued | |||||
