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麻酔下および覚醒下マウスにおけるヒゲ刺激時の賦活脳血流変化での赤血球速度と濃度の計測
https://repo.qst.go.jp/records/64530
https://repo.qst.go.jp/records/64530d99fe709-7b66-4397-ba51-32e56072b764
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-03-19 | |||||
| タイトル | ||||||
| タイトル | 麻酔下および覚醒下マウスにおけるヒゲ刺激時の賦活脳血流変化での赤血球速度と濃度の計測 | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
田桑, 弘之
× 田桑, 弘之× 松浦, 哲也× 小畠, 隆行× 川口, 拓之× 菅野, 巖× 伊藤, 浩× 田桑 弘之× 松浦 哲也× 小畠 隆行× 川口 拓之× 菅野 巖× 伊藤 浩 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Purpose: The mechanisms of regional hemodynamic response during neural activation including the concept of neurovascular coupling have been investigated using anesthetized rodent models. However, anesthesia significantly affects the physiological state including and regulation of cerebral circulation throughout the brain. The purpose of this study was to explore changes in hemodynamic characteristics during resting and sensory stimulation in awake animals as compared with those in anesthetized animals. Methods: A total of 18 male C57BL/6J mice (20-30 g, 7-11 weeks) were used in this study. Changes in CBF, red blood cell (RBC) velocity and concentration in the somatosensory cortex to whisker stimulation were measured using laser-Doppler flowmetry in awake and anesthetized mice. Results: The increase in RBC velocity observed during whisker stimulation was far higher than the increase in the RBC concentration under the awake condition ((RBC velocity: 18.4%, RBC concentration: 1.7%). 1.5% Isoflurane-induced anesthesia attenuated the increase in RBC velocity and concentration during stimulation (RBC velocity: 103%, RBC concentration: 1.4%), and the attenuation of increase in RBC velocity was quite large as compared to the change in RBC concentration. During the resting condition, significant differences in baseline CBF, RBC velocity and concentration between awake and anesthesia mice were not observed. Conclusion: Isoflurane-induced anesthesia attenuated the evoked CBF that was mainly coursed by increase in RBC velocity during stimulation, indicating the decreased in energy demand in the brain as a result of the attenuation of neuronal activity with anesthesia might inhibit the evoked RBC velocity and concentration. The RBC measurement techniques in awake animals are likely useful for the investigation of the hemodynamic changes caused by changes in neural activity or other chemical factors like PaCO2. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第37回日本微小循環学会 | |||||
| 発表年月日 | ||||||
| 日付 | 2012-03-17 | |||||
| 日付タイプ | Issued | |||||
