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64Cu-ATSM Imaging for Cancer Stem Cell-rich Regions: In Vivo and In Vitro characterization
https://repo.qst.go.jp/records/64409
https://repo.qst.go.jp/records/644097adb5be8-6a69-4f32-97e4-dad01ded3f62
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-11-22 | |||||
| タイトル | ||||||
| タイトル | 64Cu-ATSM Imaging for Cancer Stem Cell-rich Regions: In Vivo and In Vitro characterization | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yoshii, Yukie
× Yoshii, Yukie× Furukawa, Takako× Asai, Tatsuya× Kudo, Takashi× Okazawa, Hidehiko× Fujibayashi, Yasuhisa× et.al× 吉井 幸恵× 古川 高子 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | ntroduction: Cancer stem cells are recently noticed to contribute to tumor malignant behaviors, such as resistance to therapy and metastasis ability in tumors. On the other hand, it has been also known that tumor hypoxia is associated with such tumor malignancy. This indicates that tumor hypoxia might be a specific environment to keep up cancer stem cells within tumors. In this study, we examined relationships between existence of cancer stem cells and accumulation of hypoxia imaging agent 64Cu-diacetyl-bis (N4-methylthiosemicarbazone) (64Cu-ATSM) in vivo and conducted in vitro characterization. Methods: Double-tracer autoradiography and immunohistchemistry was performed with mouse colon carcinoma (Colon-26) tumor-bearing mice. In autoradiography, mixture of 74 MBq of 18FDG and 0.37 MBq of 64Cu-ATSM was intravenously injected. The distribution of radio-labeled tracers was compared with the immunohistochemical staining for CD133 expression, which reflected the characteristic of cancer stem cells in Colon-26 cells. Additionally, 64Cu-ATSM uptake and survival of CD133+ cells under hypoxia was also examined with Colon-26 cells in vitro. Results: In Colon-26 tumors, 64Cu-ATSM localizes preferentially in regions with a high density of CD133+ cells. Density of CD133+ cells was highest in regions of high 64Cu-ATSM uptake and lowest in regions of high uptake of 18FDG. In addition, we found that in vitro culturing of Colon-26 cells under hypoxia increased both 64Cu-ATSM uptake and the proportion of CD133+ cells present. Conclusion: Our findings show that, in Colon-26 tumors, 64Cu-ATSM accumulates in CD133+ cell-rich regions and that these cells would be resistant to hypoxic environment. Therefore, 64Cu-ATSM could be a potential imaging agent for cancer stem cell-rich regions within tumors. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | World Molecular Imaging Congress | |||||
| 発表年月日 | ||||||
| 日付 | 2009-09-26 | |||||
| 日付タイプ | Issued | |||||
