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腫瘍内の放射線抵抗性細胞に対する重粒子線の効果と検討
https://repo.qst.go.jp/records/64035
https://repo.qst.go.jp/records/64035d9665bb7-eb8e-466f-ab74-d1f9befa237d
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-09-30 | |||||
| タイトル | ||||||
| タイトル | 腫瘍内の放射線抵抗性細胞に対する重粒子線の効果と検討 | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
長谷川, 正俊
× 長谷川, 正俊× 浅川, 勇雄× 玉本, 哲郎× 大野, 達也× 石内, 勝吾× 加藤, 真吾× 中野, 隆史× 村上, 健× 浅川 勇雄× 大野 達也× 加藤 眞吾× 村上 健 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The purpose of this study is to investigate the radiobiological effect of heavy ion beames on intratumoral radioresistant and radiosensitive human tumor cells. Tumors of human origin, an ependymoblastoma (EB) with wild-type (wt) p53, a primitive neuroectodermal tumor (PNET) with wt p53, and a glioblastoma (GB) with mutant-type (mt) p53, were transplanted to nude mice, and the mice were irradiated with carbon ion beams (290MeV/u) or 200kV X-rays. Tumors were excised 4, 6, or 24 hours after 2Gy irradiation. A part of each tumor was fixed in formalin and embedded in paraffin. Thin sections were stained with H.E., GFAP or TUNEL for microscopic study. The other part of each tumor was stored in RNA stabilization solution, and total RNA was extracted for cDNA microarray analysis. Furthermore, some other part of the EB was frozen immediately and two types of tissue sample (A: tumor cells around vessels and B: tumor cells in the other area) were obtained using laser capture microdissection, and their RNA was extracted for cDNA microarray analysis. In wt-p53 tumors, apoptosis increased 6 hours after irradiation, and then GFAP-positive rate increased. The tumors with wt-p53 showed significant changes in gene expression following irradiation. Pathway analysis of up- and down-regulated genes demonstrated that apoptosis, p53 signaling pathway, and cell cycle are involved significantly. There was little difference between the gene expression profiles induced by carbon ion beams and those by X-rays. But, the two types of tumor cells in the EB showed different gene expression profiles following irradiation. In contrast, the tumor with mt-p53 showed much less change in gene expression after 2Gy irradiation. However, the tumor showed significant change following 8Gy or 16Gy irradiation, and the gene expression profiles induced by carbon ion beams and those by X-rays were different. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | H21年度HIMAC共同利用研究成果発表会 | |||||
| 発表年月日 | ||||||
| 日付 | 2010-04-20 | |||||
| 日付タイプ | Issued | |||||
