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[18F]FDG-PET STUDIES OF HETEROTOPIC HEART TRANSPLANTATION IN RATS
https://repo.qst.go.jp/records/64022
https://repo.qst.go.jp/records/6402227d7a3df-90d3-4ddd-b46f-e51acc47e08e
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-09-27 | |||||
| タイトル | ||||||
| タイトル | [18F]FDG-PET STUDIES OF HETEROTOPIC HEART TRANSPLANTATION IN RATS | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Inubushi, Masayuki
× Inubushi, Masayuki× Hatayama, Naoyuki× Nan, Jin Yong× Koizumi, Mitsuru× Saga, Tsuneo× et.al× 犬伏 正幸× 畑山 直之× 金 永男× 小泉 満× 佐賀 恒夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We developed a novel hyperbarically desiccated preservation method of transplants to cope with the global donor shortage, and have reported that in rats with our method the preservation time could be extended to 72 hours when resumption of beating after cardiac transplantation was defined as successful preservation. The purpose of this study was to investigate the effects of preservation time on myocardium using [18F]FDG-PET. \nMale Lewis rats were used. The hearts were extracted from donor rats, and were immediately exposed to hyperbaric mixed gas (2100 hPa PO2 + 1400 hPa PCO in study group [n=8], or 1400 hPa PO2 + 2100 hPa PCO in negative control [NC] group [n=3]) at 4DegreeC. After 24 or 48 hours of the preservation, the hearts were immersed in physiological saline, and were transplanted to the right neck of recipient rats. The rats received a cardiac transplant immediately after extraction without preservation served as positive control (PC) group (n=7). Within 2 hours after completion of the transplant surgery, the rats were injected with 37 MBq of [18F]FDG intravenously, and underwent PET imaging using a small-animal PET scanner (Siemens Inveon). [18F]FDG-PET imaging was repeated 1 week later. For semiquantitative PET analysis, ratios of the maximal counts of the donor heart (D) to the recipient heart (R) were calculated. \nIn NC and 48-hour preserved study groups, the [18F]FDG uptake to the donor hearts was low (D/R was 16.8 % and 21.0+/-3.4 %, respectively). On the other hand, the tracer uptake to the donor hearts in 24-hour preserved study group was as high as that in PC group (D/R was 119.3+/-41.9 % and 79.2+/-20.0 %, respectively), although it decreased 1 week later in the both groups (D/R was 60.0+/-26.9 % and 54.8+/-17.8 %, respectively). \n[18F]FDG-PET studies revealed that with our novel method preservation for up to 24 hours could maintain cell viability of the donor hearts as much as the immediate transplantation. However, the cell viability declined 1 week later not only in the preserved group but also in the immediate transplantation group, which suggested limitation of this animal model in evaluating long-term outcome of the transplants. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | World Federation of Nuclear Medicine and Biology 2010 | |||||
| 発表年月日 | ||||||
| 日付 | 2010-09-23 | |||||
| 日付タイプ | Issued | |||||
