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DNA double strand break repair under high and low LET radiation
https://repo.qst.go.jp/records/62881
https://repo.qst.go.jp/records/6288130af8b58-290d-4111-97c2-adb196e7aeb1
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-11-25 | |||||
| タイトル | ||||||
| タイトル | DNA double strand break repair under high and low LET radiation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Okayasu, Ryuichi
× Okayasu, Ryuichi× Kato, Takamitsu× Sekine, Emiko× Okada, Maki× Okabe, Atsushi× 岡安 隆一× 加藤 宝光× 関根 絵美子× 岡田 真希× 岡部 篤史 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | High LET heavy ion radiation has been successfully used for an alternative radiation treatment for cancer and is also an important component for space radiation. The biological effectiveness of high LET radiation (up to 200keV/um) is, in general, higher than that of low LET radiation such as X-rays and r-rays. One of the main causes of this is thought to be the difference in the way how cells process DNA damage, particularly DNA double strand breaks (DSBs). We have recently shown that the repair of DSBs depends on LET values, and DSB repair deficient cells show less dependency on LETs for their cell survival as well as DSB repair process. The inefficient repair of DSB associated with high LET radiation was also confirmed by a sensitive method, rH2AX assay. This inefficiency seems to result in misrejoining of chromosomes, leading to further severe biological consequences such as cell death and mutation. The misrepair/misrejoining could be shown by fluorescence in situ hybridization (FISH) of chromosomes in irradiated cells. \nThe phosphorylation and de-phosphorylation of DNA-PKcs, a key protein for non-homologous end joining (NHEJ), was significantly delayed by irradiation of high LET carbon and iron ions when compared to X-rays. Of interest, this phenomenon was further accentuated in the human NHEJ defective 180BR cells (shown below, Ref.: Radiat. Res. 165: 59-67 (2006)) |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第7回 放射線生物学に関する日仏ワークショップ | |||||
| 発表年月日 | ||||||
| 日付 | 2008-10-16 | |||||
| 日付タイプ | Issued | |||||
