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Current status of microbeam irradiation system for mammalian cells, SPICE at NIRS
https://repo.qst.go.jp/records/62847
https://repo.qst.go.jp/records/62847b0da814a-4f8b-40c1-8960-162cfda76bc0
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-11-11 | |||||
| タイトル | ||||||
| タイトル | Current status of microbeam irradiation system for mammalian cells, SPICE at NIRS | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Konishi, Teruaki
× Konishi, Teruaki× Iso, Hiroyuki× Ishikawa, Takahiro× Yasuda, Nakahiro× Oikawa, Masakazu× Suya, Noriyoshi× Higuchi, Yuichi× Kodama, Kumiko× Katou, Takeshi× Hafer, Kurt× Hamano, Tsuyoshi× Kitamura, Hisashi× Hieda, Kotaro× Imaseki, Hitoshi× 小西 輝昭× 磯 浩之× 石川 剛弘× 安田 仲宏× 及川 将一× 酢屋 徳啓× 樋口 有一× 児玉 久美子× 加藤 武伺× Ｈａｆｅｒ Ｋｕｒｔ× 濱野 毅× 北村 尚× 檜枝 光太郎× 今関 等 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | INTRODUCTION Single-cell microbeam irradiation systems have become significant tools in the field of radiation biology. Recently, many microbeam facilities have been developed, and are available for biological research. Also in Japan, there are many microbeam facilities with different types of radiation sources that are now available for biological studies, such as low-dose effects, hyper radio-sensitivity, bystander effects and others. The single particle irradiation system (SPICE) 1) of National Institute of Radiological Sciences (NIRS) gener-ates 3.4 MeV protons with an approximately 5 um beam, and is the only microbeam irradiation system in Japan with low-LET particles irradiation. We will describe the specification of SPICE in 5 separate poster presentations, 0. Outline of Tandem accelerator facili-ties in NIRS, 1. Accelerator and beam line optics, 2. Beam exit window and calculation on beam focusing, 3. Micro-scope and cell targeting system, and 4. Preliminary biologi-cal results. \nSPECIFICATION OF SPICE Accelerator and beam line optics The Electrostatic accelerator facility of NIRS supplies protons and helium ion beam by a Tandetron accelerator. In this facility, there are four beam lines, and three horizontal beam lines are available for PIXE analysis PASTA2). Forth beam line, SPICE is a vertical beam line, the beam of which is transported upward after a 90 degrees bending magnet installed in the middle of the microbeam scanning PIXE beam line. Characteristic of SPICE is that a beam is focused by two slit system and a Q-magnet lens(Oxford Microbeam Ltd.)so as to exclude such low-energy particle components by scattering seen in other microbeam facilites produced by the collimation method. Outline of beam line, 90 degrees bending magnet, slits, and Q-magnet will be introduced in the presentation. \nBeam exit window and calculation on beam focusing We accomplished to irradiate approximately 100~500 protons within the area of the 5 um diameter Beam size was measured by plastic track detector, CR-39 (HARZLAS TD-1). A CR-39 of 100 um thick was adhered to cell dish instead of cells, and then the dish was set on the voice coil motor stage (Fig 1). However, the scattering of proton beam takes place drastically when the protons pass through the 1µm thick Si3N4 film, which is used as beam exit window and the air gap between the beam window and the targeted cell. Result of calculations on the beam scattering with dif-ferent parameters such as window materials, its thickness, air gap, using SRIM2008 code and results of the beam pro-file measurements using CR-39 will be presented. \nMicroscope and cell targeting system Our cell targeting system captures all images of the dyed cell nuclei with fluorescent microscope equipped with CCD camera and computes the X-Y coordinates of the cell posi-tion according to the fluorescence. All of the irradiation procedures could be performed automatically after setting some parameters, such as a preset number of protons. The number of proton traveling through the cells was counted using a scintillation detector equipped on microscope system, which is set above the cell dish. A fast beam deflector was used to switch off the beam on demand, which was installed upstream of the 90-degree bending magnet. Computer that controls the voice coil motor stages gives a high-speed trig-ger pulse to the beam deflector to turn on/off the beam. It is possible to irradiate from a single to an arbitrary number of protons per position or cell. Specifications of microscope, voice coil motor stage, and cell recognition software will be presented. \nPreliminary biological results: To confirm whether the targeted cells were accurately irradiated, firstly we applied the cell relief embossment tech-nique using CR-39 to visualize cell image and the beam as etch pits, simultaneously3). Secondly, we visualized the phosphorylated histone protein, gamma-H2AX, which is known as a marker for DNA double strand breaks by immuno-staining the cells with antibody. From these two results we demon-strated that all the targeted cells are actually irradiated. We also achieved a result showing the production of intra-cellular reactive oxygen species using DCF-DA as a fluo-rescent marker in irradiated CHO-K1 cells 4). Preliminary result on cell inactivation will also be presented. \nCONCLUSION Now we are focusing on low dose effects and how to ob-tain survival curves to measure hyper-radio sensitivity by irradiating CHO-K1 cell lines and its DNA repair deficient cell lines. SPICE is currently operative and open facility for biological studies. \nREFERENCES 1) Imaseki H. et al., Nucl Inst Meth B 260; 81, 2007 2) Yamaguchi H. et al., Nucl Inst Meth B, 210: 292, 2003 3) Konishi T. et al., J Radiat Res, 48: 255, 2007 4) Hafer K. et al., Radiat Res, 169: 469, 2008 \n*Corresponding author: Teruaki KONISHI E-mail: tkonishi@nirs.go.jp 1 Natl Inst Radiol Sci, Chiba 263-8555 Japan 2 Neos-Tech Co., Ltd., Chiba, 206-0045 Japan 3 Rikkyo University, Tokyo, 171-8501, Japan |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 8th International Workshop on Microbeam Probes of Cellular Radiation Response | |||||
| 発表年月日 | ||||||
| 日付 | 2008-11-15 | |||||
| 日付タイプ | Issued | |||||
