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Chromosome aberrations in the mouse spleen cells exposed to low dose (rate) radiation

https://repo.qst.go.jp/records/62767
https://repo.qst.go.jp/records/62767
8ac5ae88-c408-46a5-bd19-e1ae7e207842
Item type 会議発表用資料 / Presentation(1)
公開日 2008-10-08
タイトル
タイトル Chromosome aberrations in the mouse spleen cells exposed to low dose (rate) radiation
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_c94f
資源タイプ conference object
アクセス権
アクセス権 metadata only access
アクセス権URI http://purl.org/coar/access_right/c_14cb
著者 Yoshida, Mitsuaki

× Yoshida, Mitsuaki

WEKO 619880

Yoshida, Mitsuaki

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Akiyama, Miho

× Akiyama, Miho

WEKO 619881

Akiyama, Miho

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Minamihisamatsu, Masako

× Minamihisamatsu, Masako

WEKO 619882

Minamihisamatsu, Masako

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Yoshida, Kazuko

× Yoshida, Kazuko

WEKO 619883

Yoshida, Kazuko

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Hayata, Isamu

× Hayata, Isamu

WEKO 619884

Hayata, Isamu

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Ono, Tetsuya

× Ono, Tetsuya

WEKO 619885

Ono, Tetsuya

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吉田 光明

× 吉田 光明

WEKO 619886

en 吉田 光明

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穐山 美穂

× 穐山 美穂

WEKO 619887

en 穐山 美穂

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南久松 眞子

× 南久松 眞子

WEKO 619888

en 南久松 眞子

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吉田 和子

× 吉田 和子

WEKO 619889

en 吉田 和子

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早田 勇

× 早田 勇

WEKO 619890

en 早田 勇

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小野 哲也

× 小野 哲也

WEKO 619891

en 小野 哲也

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抄録
内容記述タイプ Abstract
内容記述 In order to investigate the biological effects of low dose radiation, the
chromosomes were analyzed in the spleen cells from the mice exposed to low dose rate
radiation for a long term. The Fl mice between gpt-delta transgenic and SWR strains were irradiated at the three kinds of dose rate (average 0,04255 mGy/day, 0.858 mGy/day and 16.56 mGy/day) of y-ray for 483 days. The total doses in these mice were approximately 20 mGy, 400 mGy and 8 Gy. The spleen tissues were disaggregated by squeezing and the lymphocytes were collected and cultured for about 46 - 48 hrs in the20% FCS-RPMI1640 medium containing Concanavarin A and Lipopolysaccharides.
Colcemid was added to the culture for 24 hours before starting the harvest. Mitotic cells
were obtained from 6, 7, 8 and 6 mice exposed to 0 mGy (control), 20 mGy, 400 mGy and 8 Gy, respectively. In the dicentric analysis, we counted only the typical dicentric chromosome due to the reciprocal reunion of the terminal regions of two chromosomes.
The frequency of a dicentric chromosome per metaphase was 0.0004, 0.0013, 0.0022
and 0.023 in mice exposed to 0 mGy, 20 mGy, 400 mGy and 8 Gy, respectively. We also analyzed dicentric chromosomes in splenic lymphocytes from the mice received acute irradiation (dose rate: 0.72 Gy/min). The Fl mice as mentioned above were acutely irradiated at four doses; 0.4 Gy, 1.0 Gy, 2.0 Gy and 4.0 Gy. The metaphases suitable for the analysis were obtained from all the mice except for the mice irradiated at 4.0 Gy. The frequencies of typical dicentric chromosomes were 0, 0.02, 0. 14 and 0.43 in mice received 0 Gy (control), 0.4 Gy, 1.0 Gy and 2.0 Gy, respectively. The dose dependency was clearly observed in both studies, although the dose rate was different in the study of low dose rate irradiation. The results in the present study also prove that the chromosome aberration may be the most valuable and sensitive biomarker to investigate the effect of low dose (rate) radiation.
会議概要(会議名, 開催地, 会期, 主催者等)
内容記述タイプ Other
内容記述 New Nuclear Research Symposium "Biological Responses to Low Dose Radiation"
発表年月日
日付 2007-11-13
日付タイプ Issued
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